Nikon Eclipse 80i Research Fluorescence Microscope with Digital Imaging Head and CFI60 Optics

The Nikon Eclipse 80i is a versatile research-grade fluorescence microscope designed for both brightfield microscopy and multi-channel epi-fluorescence imaging. Equipped with Nikon’s CFI60 infinity optical system, it integrates a 12V 100W halogen transmitted-light source alongside a 75W xenon high-pressure arc fluorescence light source, facilitating high-quality imaging for biomedical engineering, synthetic biology, and molecular diagnostics applications.

Key technical features include:

• Epi-fluorescence illumination with a six-position manual filter turret supporting installed filter cubes for *FITC* (green, 465-495nm excitation), *TRITC* (orange/red, 540-550nm excitation), and *Cy5* (far-red, 620-650nm excitation), enabling multi-channel fluorescence analyses essential in gene editing and live-cell imaging workflows.
• Trinocular viewing head with adjustable inclination for ergonomic binocular and camera-based observation, critical for detailed fluorescence documentation.
• Integrated Nikon DIH digital imaging head with a six-position cube turret, built-in shutter, and optical routing, coupled with two AmScope MU500-HS 5.1 MP digital cameras and a wired remote shutter, streamlining image acquisition in molecular biology and bioprocessing research.
• Precision coaxial coarse/fine focusing with a 1µm minimum reading and focus clamp, suitable for demanding clinical diagnostics and cell imaging tasks.
• A comprehensive set of Nikon Plan Fluor objectives (4x/0.13, 10x/0.30, 20x/0.50, 40x/0.75, 60x/0.85) and neutral density filters (ND4, ND8, ND16, ND32), supporting controlled illumination for fluorescence intensity modulation critical in sensitive biopharmaceutical production pipelines.

Included with the system are all necessary power supplies, communication cables, Nikon CFI 10x/22 eyepieces, fluorescence filters, software (Amscope X64 4.12), and user manual. The microscope operates on 100-230 V (50/60 Hz) power and weighs approximately 27 lbs.

This configuration is optimized for laboratories requiring a robust platform for fluorescence-based cell imaging, molecular diagnostics, and synthetic biology experiments, providing reliable performance for advanced biotechnological research and biomedical engineering applications.