Nikon Eclipse Ti-E PFS Motorized Inverted LED Fluorescence Phase Contrast Microscope with Motorized XY Stage

The Nikon Eclipse Ti-E PFS is a sophisticated motorized inverted fluorescence microscope optimized for advanced cell imaging, biotechnology, and molecular diagnostics applications. It integrates a Perfect Focus System (PFS) for automated focal drift correction, crucial for long-term live-cell imaging, time-lapse studies, and high-precision bioprocess monitoring.

Key technical features include:

• Microscopy techniques: Brightfield with phase contrast, LED fluorescence excitation, and PFS-enabled automated focus maintenance
• Motorized components: Motorized Z-axis drive, objective turret, filter cube turret, LED fluorescence light path, and XY motorized stage with joystick control for precise specimen positioning
• Illumination and filters: Lumencor LED illumination with collimating adapter, TI-FLC-E 6-position motorized filter cube turret, and up to three new fluorescence filter sets (e.g., DAPI, FITC/EGFP, Texas Red, Cy5) supporting multiple fluorochromes essential for synthetic biology and molecular cloning workflows
• Optical components: High-quality Plan Fluor and Plan Apo VC objectives (4x, 10x, 20x) with motorized nosepiece and mechanical condenser with phase contrast inserts for enhanced contrast of transparent samples
• Imaging system: Integrated Bestscope 8.3MP color CMOS camera, coupled with open-source Micromanager and ImageJ software for multi-dimensional image acquisition (multi-slice Z-stacking, multi-position stitching, multi-frame time-lapse) enabling quantitative fluorescence analysis and biomanufacturing research
• Focus system: Nikon’s PFS uses infrared light for continuous focus offset detection and real-time correction, minimizing phototoxicity and photobleaching—critical for live-cell and neuronal activity studies
• Applications: Widely used in biotechnology, molecular biology, pharmacology, clinical diagnostics, embryology imaging, surface topography studies, and drug response assays due to its precise fluorescence intensity control and automated focus stabilization
• Customization and upgrades: Flexible configuration options include adding DIC or polarization modules, higher magnification objectives (up to 1000x), enhanced fluorescence light sources (Xenon, metal halide), and advanced CCD, sCMOS, or EMCCD cameras for improved resolution and sensitivity in biomedical engineering and synthetic biology workflows
• Software capabilities: Supports advanced image processing—annotation, calibration, measurement, and analysis—facilitating detailed quantification in research and biotechnology environments
• Warranty and support: Comes with a 180-day US warranty, remote training sessions, installation support, and expert servicing ensuring reliable operation in demanding research settings

This motorized inverted fluorescence microscope is essential for laboratories requiring precision live-cell imaging, automated multi-well plate scanning, and comprehensive fluorescence analysis, making it a valuable asset in bioprocessing, gene editing, and molecular cloning workflows.