Nikon Eclipse Ti-E Inverted Motorized LED Fluorescence and DIC Microscope with Motorized XY Stage

The Nikon Eclipse Ti-E is a high-performance _inverted fluorescence microscope_ designed for advanced cell imaging, live cell observation, and multidimensional fluorescence analysis widely adopted in biotechnology and molecular biology research. This motorized system integrates the following technical specifications and features:

• Microscope Base & Head:
• Nikon Eclipse Ti-E inverted base with fully motorized Z-axis positioning using TI-HUBC/A controller
• Binocular head with two eyepieces supporting multiple camera port distributions (100% eyepiece, 80% camera, 100% camera)
• Fluorescence Illumination & Filters:
• Lumencor LED light source with collimating adapter and light guide ensuring stable, uniform fluorescence excitation
• Motorized 6-position fluorescence filter cube turret (TI-FLC-E) with three newly installed filter sets selectable from: DAPI, FITC/EGFP/Cy2, Texas Red/TRITC/Cy3, Cy5 (optionally upgraded light source required for Cy5)
• Imaging & Analysis:
• Equipped with a Bestscope 8.3MP color CMOS camera for high-resolution fluorescence and brightfield capture
• Dell Windows 10 laptop with open-source Micromanager and ImageJ software providing multiframe time-lapse, Z-stack, XY stitching, image calibration, and annotation essential for live-cell imaging and biomedical engineering applications
• Optical Components and Objectives:
• Motorized 6-position nosepiece (TI-ND6) with Plan Fluor objectives: 4x/0.13, 10x/0.3, and high-quality 20x/0.75 CFI Plan Apo VC objective
• DIC components include polarizer, condenser prism, objective prism, and analyzer for enhanced contrast of unstained samples—crucial in materials science and pharmacological studies
• Stage and Control:
• Motorized XY stage with LEP MAC6000 controller and joystick enabling precise sample positioning, beneficial for multi-position fluorescence microscopy and quantitative analysis workflows
• Brightfield Illumination:
• 12V 100W Halogen lamp with illumination tower for versatile brightfield imaging and differential interference contrast (DIC) capabilities
• Automation and Benefits:
• Full automation with motorized light path, filter turret, objective turret, XY stage, and Z-axis focus allows automated parfocality adjustment, recall of saved focus positions, and fine/coarse focus control, enhancing throughput in molecular diagnostics and synthetic biology research pipelines
• Customization and Upgrades:
• Flexible configuration options include additional microscopy techniques such as phase contrast and polarization, advanced high-magnification Nikon objectives, upgraded fluorescence light sources (Xenon/Metal Halide/LED), and higher-performance CCD/sCMOS/EMCCD cameras for enhanced image sensitivity in biopharmaceutical production and genetic engineering studies
• Applications:
• Widely used in biotechnology, cell imaging, neuroscience, molecular biology, drug discovery, and biomedical research. Ideal for fluorescence labeling, time-lapse imaging, live cell dynamics, and high-resolution structural studies. Supports critical workflows in bioprocessing, gene editing, and molecular cloning
• Service and Warranty:
• Delivered fully serviced with preventive maintenance completed, optics verified for uniformity and field distortion, and fluorescence intensity calibrated. Comes with 180-day USA and 60-day international warranty, plus optional extended warranty
• Remote and onsite training for microscope operation and software provided, supporting smooth integration into research laboratories and industrial biomanufacturing environments

This microscope is a versatile tool for researchers requiring precise fluorescence microscopy combined with motorized automation, enabling reproducible and high-throughput imaging vital to cutting-edge molecular diagnostics and biotechnological innovation.