YellowSub PCR Additive 10x1 ml for enhanced PCR yield and direct gel loading

YellowSub PCR Additive is designed to improve enzymatic PCR reactions by increasing yield and specificity. It contains a specialized formulation and combines a loading buffer with a yellow dye that migrates at ~40 base pairs, facilitating direct application of the PCR mix onto agarose gels without further additions. Compatible with various thermostable polymerases (Taq, Pfu, Tth, Pfx) and restriction enzymes, it supports downstream applications such as cloning and restriction digest screening. It also integrates well with DNA purification protocols using glass milk or Qiagen spin columns.

Key Features:

• Enhances PCR yield and specificity
• Contains loading buffer and visible yellow dye for direct gel loading
• Dye migration at ~40 bp allows visualization of short DNA fragments
• Compatible with multiple thermostable DNA polymerases
• Suitable for restriction enzyme digestion and screening
• Works with standard DNA purification methods

Scientific Validation:

The product has been validated and cited in several studies and publications covering PCR specificity, polymorphic sex testing, tumor cell gene therapy research, epidemiological studies, DNA methylation assays, tick identification via PCR-RFLP, pathogen detection in buffalo, and fungal differentiation using MALDI-TOF mass spectrometry.