Invitrogen pENTR/D-TOPO

Invitrogen pENTR/D-TOPO Cloning Kit

The Invitrogen pENTR/D-TOPO Cloning Kit offers a rapid and efficient method for directionally cloning blunt-end PCR products into an entry vector, facilitating seamless integration into the Gateway™ System. This system enables researchers to easily transfer genes between various expression vectors without the need for restriction enzymes or ligation steps.

Key features and benefits:

• Rapid cloning process: Achieve directional cloning in approximately 5 minutes, streamlining molecular cloning workflows.
• High efficiency: Obtain over 90% of clones with the correct insert orientation, ensuring reliable results.
• Simplified protocol: Eliminates the need for PCR product purification, vector preparation, or additional enzymatic treatments.
• Versatile compatibility: Seamlessly integrates with the Gateway™ System, allowing for easy shuttling of cloned genes between multiple vector systems.
• Comprehensive kit components: Includes the pENTR/D-TOPO vector, dNTPs, salt solution, sterile water, and universal M13 sequencing primers, providing all necessary reagents for successful cloning.

This cloning kit is widely utilised in various scientific fields, including molecular biology, genetics, biotechnology, and biomedical research. It is commonly employed in university laboratories, biomedical research centres, and biotechnology companies for applications such as gene expression studies, protein production, and functional analysis.

First introduced over a decade ago, the Invitrogen pENTR/D-TOPO Cloning Kit continues to be a valuable tool in molecular cloning, offering a reliable and efficient solution for researchers in the biotechnology industry.