Cytena UP.SIGHT Dual-Imaging Automated Single-Cell Dispenser with Integrated Microscopic Verification

The Cytena UP.SIGHT Dual-Imaging Single-Cell Dispenser is a benchtop instrument engineered for automated, gentle microfluidic dispensing of single cells into microplates, optimized for applications in cell line development, single-cell cloning, and molecular diagnostics. This advanced system integrates dual imaging verification using CMOS cameras at both the nozzle (0.81 MP, 10x magnification, 3 µm optical resolution, 488 nm excitation) and well level (13.4 MP, 5x magnification, 2.2 µm optical resolution) to confirm accurate single-cell deposition and clonality assurance.

Key technical features include:

• Dual-Imaging Cell Verification: Confirms cell presence at dispensing nozzle and verifies deposition within destination wells, critical for monoclonality assurance and colony tracking.
• 3D Full Well Imaging: Captures the complete well volume optically without stitching, enabling detailed fluorescence and brightfield imaging for biologics and therapeutic protein research.
• Microfluidic Dispensing Technology: Uses piezo-driven disposable cartridges to isolate single cells gently, minimizing stress and contamination risk.
• Automated Motorized Plate Positioning: Supports dispensing into standard 96- and 384-well plates with precise well bottom imaging and minimal processing time (~2 min for 96-well dispensing).
• Embedded Imaging and Control Software: Provides real-time cell analysis with on-board computational power for responsive workflow management.
• Compact Footprint and Integrated Optics: Suitable for biotechnology labs focused on gene editing, synthetic biology, rare cell isolation, and biopharmaceutical production pipelines.

Performance Specifications:

• Single-cell dispensing times: ~2 minutes per 96-well plate; ~8 minutes per 384-well plate.
• Weight: 40 kg; power input 100-240 VAC, 50/60 Hz.
• Imaging file format: JPEG; excitation wavelength 488 nm, emission 520 ± 36 nm.
• Verified accurate cell counting and focusing using microbead standards (3 µm and 10 µm beads).

This instrument is widely adopted in synthetic biology research and molecular cloning workflows where high precision and monoclonality assurance are critical. The disposable cartridge system and automated imaging support rigorous molecular diagnostics and bioprocessing applications, making it integral to biomanufacturing pipelines.

The Cytena UP.SIGHT is supplied fully tested with warranty, ensuring functionality of optical assemblies, dispensing accuracy, imaging responsiveness, and safety features. It supports reliable operation in clinical diagnostics, cell imaging, and live-cell dispersion protocols.

For biotechnology professionals and researchers, the UP.SIGHT system facilitates efficient rare cell isolation and single-cell dispensing with high-throughput capability, enabling advanced applications in biomedical engineering and protein therapeutics development.