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Eppendorf 5427R Refrigerated Benchtop Microcentrifuge with FastTemp Cooling

UsedEppendorfCentrifugesUnited States

Price on request

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Capacity
48 x 1.5/2.0 mL, 12 x 5.0 mL
Max. Speed
16,220 rpm
Max. RCF
25,001 x g
Temperature Range
-11 °C to 40 °C
FastTemp function for quick pre-cooling
11 minutes from ∼23°C to 4°C
All 11 specifications
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Specifications 11

Brands
Eppendorf
New/Used
Used
Capacity
48 x 1.5/2.0 mL, 12 x 5.0 mL
Max. Speed
16,220 rpm
Max. RCF
25,001 x g
Temperature Range
-11 °C to 40 °C
FastTemp function for quick pre-cooling
11 minutes from ∼23°C to 4°C
Noise Level
< 55 dBA with rotor FA-45-48-11
Dimensions
12x20x10”
Weight
72lbs
Power
120v, 50/60Hz, 495w

Product description

Refrigerated microcentrifuge 5427R delivers up to 16,220 rpm, 25,001 x g, temp range -11 to 40 °C for biotech workflows.

The Eppendorf 5427R is a high-performance refrigerated benchtop microcentrifuge designed for precise temperature control and enhanced speed, making it essential for molecular diagnostics, bioprocessing, and synthetic biology applications. With a maximum speed of 16,220 rpm and relative centrifugal force (RCF) reaching 25,001 x g, this microcentrifuge efficiently processes samples in tubes up to 2.0 mL capacity, accommodating 48 tubes simultaneously depending on rotor configuration.

*Key technical features include:*

  • Temperature range: from -11 °C to 40 °C, critical for temperature-sensitive protocols in biotechnology and clinical diagnostics
  • FastTemp function: rapidly cools the chamber from approximately 23 °C to 4 °C in just 11 minutes, supporting fast pre-cooling requirements in live-cell imaging and gene editing workflows
  • Compact benchtop footprint: dimensions 12 x 20 x 10 inches and weight of 72 lbs optimize bench space in high-throughput biomedical labs
  • Quiet operation: noise level below 55 dBA for a low-disturbance environment during prolonged runs
  • Flexible timing: programmable runs from 10 seconds to 9 hours 59 minutes, suitable for diverse cell imaging and biomanufacturing sample preparations
  • Capacity: supports 48 x 1.5/2.0 mL tubes or 12 x 5.0 mL tubes, catering to microtube centrifugation pathways critical in synthetic biology research

The Eppendorf 5427R refrigerated centrifuge is widely adopted for its reliability and precision in molecular biology workflows, ensuring consistent sample integrity and functional temperature control. Its latch-less lid simplifies sample handling while maintaining user safety and operational efficiency. This model is highly valued in laboratories focusing on biotechnology, molecular cloning, and clinical research applications, offering robust performance in a compact design ideal for temperature-sensitive sample processing.

Why Questpair exists

Lab equipment markets are fragmented. Buyers spend weeks calling distributors, comparing quotes, and verifying supplier reliability. Sellers struggle to reach global research labs.

We built Questpair to fix this. Every supplier on the platform is verified before listing. Every quote you receive comes from a real, vetted seller. AI prepares your case in minutes, then a real specialist takes over.

We're an independent team based in The Netherlands, working with research labs and procurement teams across Europe and worldwide.

Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Yes. Every verified supplier states which service partners cover the instrument, and we list that alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Can't find the exact configuration?

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Last updated 4 September 2026