Oxion OX.2453-PLF Inverted Trinocular Fluorescence Microscope with Mechanical Stage

The Oxion OX.2453-PLF is a high-precision inverted trinocular fluorescence microscope designed for advanced fluorescence analysis and live-cell imaging in biotechnology and clinical diagnostics. This model features:

• Optics:
• Wide-field planar eyepieces (HWF 10x / 22 mm) with DIN standards for expanded field of view.
• Quintuple internal revolving nosepiece equipped with long working distance (LWD) Plan Fluarex objectives:
• 10x/0.30 (bright field, infinity corrected),
• 20x/0.45 and 40x/0.65 (phase contrast, infinity corrected).
• All lenses include anti-fungal and anti-reflective coatings to maximize light transmission—critical for sensitive fluorescence microscopy applications.

• Mechanical Stage and Focus:
• Mechanical XY stage (120 x 78 mm travel) on a 250x230 mm platform for precise specimen positioning.
• Macro and fine coaxial focus knobs with 2 μm precision and ~24 mm travel enable meticulous focal adjustments.

• Condenser:
• Numerical aperture (NA) 0.30 condenser with iris diaphragm and 72 mm working distance.
• Equipped with phase contrast slider compatible with 4x, 10x, 20x, and 40x objectives.

• Fluorescence Illumination System:
• 4-position filter turret with critical filter blocks tailored for multichannel fluorescence:
• Blue: EX 465–495 nm BP, DM 505 nm LP, EM 515–558 nm BP
• Green: EX 520–600 nm BP, DM 600 nm LP, EM 605–665 nm BP
• UV: EX 361–381 nm BP, DM 415 nm LP, EM 435–495 nm BP
• Transmitted illumination via 5W LED with adjustable intensity and internal transformer supporting 100–240 V supply—a robust setup for fluorescence excitation and transmitted light contrast enhancement.

• Head Assembly:
• Trinocular head inclined at 45°, interpupillary distance adjustable from 54 to 75 mm.
• Vertical trinocular port with 100:0% / 80:20% image split for simultaneous photography or video capture.

• Additional Features and Accessories:
• Includes metal culture dish stage insert (Ø 35 mm), specimen holder for 76x26 mm slides, and glass plate.
• Comes with carrying case, dust cover, power cable, and instruction manual.

This instrument is widely adopted in molecular diagnostics, bioprocessing, and synthetic biology research environments where precise fluorescence imaging and phase contrast microscopy are essential. Its long working distance objectives and customizable filter sets make it especially suitable for live-cell imaging and fluorescence analysis workflows in biotechnology and biomedical engineering.

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