MilliporeSigma Amicon Ultra

The Amicon® Ultra 15 mL Centrifugal Filter Unit is a high-performance ultrafiltration device designed for the rapid concentration and purification of biological samples. Utilising a 30 kDa molecular weight cut-off (MWCO) Ultracel® regenerated cellulose membrane, this filter efficiently retains macromolecules while allowing smaller molecules to pass through, making it ideal for applications in protein purification, enzyme concentration, and nucleic acid desalting.

Key features include:

• High recovery rates: Achieves retentate recovery of greater than 90%, ensuring minimal sample loss.

• Rapid processing times: The vertical membrane design reduces concentration polarization, enabling spin times as fast as 10–15 minutes.

• High concentration factors: Capable of achieving 80- to 100-fold concentration, facilitating efficient sample processing.

• User-friendly design: Features a translucent housing with volume gradations for easy sample monitoring and direct pipette access to the concentrate, eliminating additional processing steps.

• Broad chemical compatibility: Compatible with a variety of aqueous solutions across a pH range of 1 to 9, enhancing versatility in different laboratory settings.

This centrifugal filter is widely used in biomedical research, molecular biology, and clinical diagnostics for applications such as:

• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.

• Purification of macromolecular components from tissue culture extracts or cell lysates.

• Desalting, buffer exchange, and protein dialysis.

Commonly employed in university laboratories, biomedical research centres, and clinical environments, the Amicon® Ultra 15 mL Centrifugal Filter Unit continues to be a reliable tool for researchers seeking efficient and high-yield sample preparation.