MilliporeSigma Amicon Ultra-15

Amicon® Ultra-15 Centrifugal Filter Units are designed for the rapid concentration and purification of biological samples, including proteins, nucleic acids, and other macromolecules. These devices are widely used in various scientific fields for sample preparation prior to analysis.

Key features and benefits:

• High-recovery Ultracel® regenerated cellulose membranes with molecular weight cut-offs (MWCOs) of 3, 10, 30, 50, or 100 kDa, ensuring greater than 90% retentate recovery.
• Vertical membrane design reduces concentration polarization, enabling ultrafast spin times as short as 10–15 minutes.
• Heat-sealed membranes minimize downstream extractables, ensuring sample purity.
• Universal rotor compatibility allows use with both swinging bucket and fixed-angle rotors.
• High concentration factors of 80- to 100-fold, with a minimum final concentrate volume of 200 μL.
• Translucent housing with volume gradations facilitates easy sample monitoring.
• Direct pipettor access to the sample reservoir eliminates additional processing steps to recover the concentrate.

These centrifugal filter units are extensively applied in:

• Proteomics for concentrating dilute protein solutions and purifying macromolecular components from cell lysates.
• Genomics for nucleic acid purification and concentration.
• Clinical diagnostics for concentrating serum, urine, cerebrospinal fluid, and other body fluids prior to analysis, including testing for Bence Jones protein to diagnose multiple myeloma.

Commonly utilised in university laboratories, biomedical research centres, and clinical diagnostic facilities, these devices have been integral to sample preparation protocols since their introduction and continue to be relevant in modern research and diagnostic applications.