MilliporeSigma Amicon Ultra-0.5

Amicon® Ultra-0.5 centrifugal filter devices are designed for rapid and efficient concentration and purification of biological samples, making them indispensable in biotechnology and molecular biology laboratories.

Key features:

• High concentration capability: Concentrates 500 µL samples down to 15 µL, achieving a 25x to 30x concentration factor.
• Superior sample recovery: Typically recovers over 90% of the initial sample, ensuring minimal loss of valuable biomolecules.
• Rapid processing: Completes ultrafiltration in 10 to 30 minutes, enhancing laboratory efficiency.
• Reverse spin design: Facilitates consistent and efficient recovery of concentrated samples.
• Versatile membrane options: Available with molecular weight cutoffs (MWCO) ranging from 3 kDa to 100 kDa, accommodating various molecular sizes.

Applications:

• Protein concentration and purification: Ideal for concentrating enzymes, antibodies, and other proteins.
• Nucleic acid purification: Effective in concentrating and purifying DNA and RNA samples.
• Buffer exchange and desalting: Facilitates rapid buffer exchange and removal of small contaminants.
• Sample preparation for HPLC: Removes proteins and other macromolecules prior to high-performance liquid chromatography.

Commonly used in:

• Biotechnology companies: For bioprocessing and biomanufacturing applications.
• Academic research laboratories: Supporting studies in molecular biology and synthetic biology.
• Clinical research facilities: Assisting in molecular diagnostics and other clinical applications.

Introduced prior to 2010, these filters continue to be widely used in laboratories worldwide, reflecting their enduring relevance and reliability in scientific research.