Eppendorf 5420 Non-Refrigerated Microcentrifuge for Molecular Biology

The Eppendorf 5420 is a compact, high-performance non-refrigerated microcentrifuge designed for molecular biology and biotechnology laboratories. It is particularly optimized for spin-column based DNA/RNA purification, gel extraction, and PCR sample preparation workflows essential in molecular diagnostics and synthetic biology research.

Key technical specifications include:

• Compact footprint: 24 × 35 × 22 cm (9.4 × 13.8 × 8.7 in), suitable for space-limited benchtops in clinical diagnostics and bioprocessing labs.
• Maximum speed: 15,060 rpm generating up to 21,300 × g centrifugal force, enabling efficient pellet formation and nucleic acid isolation.
• Rotor capacity: Aerosol-tight rotor holding 24 × 1.5/2.0 mL tubes standard; with adapters for 0.2, 0.4, 0.5 mL tubes, 0.6 mL BD Microtainers; and a rotor for 32 × 0.2 mL PCR tubes or four 8-strip PCR sets, enhancing flexibility for various sample formats.
• Ergonomic operation: Soft-touch lid closure with one-finger operation and automatic lid opening post-run reduces sample warming—critical for sensitive biomolecule integrity. Includes a one-push short-spin function for rapid pelleting.
• Safety features: Aerosol-tight rotor minimizes biohazard risks, supporting safe handling of pathogenic or hazardous samples commonly encountered in biomedical engineering and molecular cloning workflows.
• Noise level: Quiet operation below 56 dB(A), enabling use in shared laboratory environments.
• Run timer: Programmable from 10 seconds up to 9 hours 59 minutes plus continuous mode, suitable for diverse protocol requirements.

This microcentrifuge is widely used in biotechnology research, molecular biology, and clinical diagnostics laboratories for reliable centrifugation of nucleic acids and PCR reaction components. Its aerosol containment makes it critical in workflows involving gene editing and biopharmaceutical production pipelines where sample safety and contamination prevention are paramount. The Eppendorf 5420 is an essential tool for laboratories performing high-throughput molecular assays and bioprocessing applications requiring consistent centrifugation performance in a compact form factor.