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Eppendorf 5420 Benchtop Microcentrifuge with FA-24×2 Rotor

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Maximum RCF
21,300 × g
Maximum Speed
100 – 15,060 rpm
Maximum Capacity
24 × 1.5/2.0 mL
Acceleration Time
15 s
Deceleration Time
15 s
All 13 specifications
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Specifications 13

Brands
Eppendorf
New/Used
Used
Maximum RCF
21,300 × g
Maximum Speed
100 – 15,060 rpm
Maximum Capacity
24 × 1.5/2.0 mL
Acceleration Time
15 s
Deceleration Time
15 s
Timer
10 s to 99:59 h, with continuous run function
Weight
45 lbs
Dimensions
10 x 14 x 9”
Power
120V 50/60Hz
Max Power Consumption
270 W
Noise Level
< 56 dB(A)

Product description

Compact benchtop microcentrifuge, 15,060 rpm and 21,300 × g, for DNA, RNA, and PCR sample processing.

The Eppendorf 5420 is a compact benchtop microcentrifuge designed specifically for routine molecular biology workflows, including molecular cloning, PCR preparation, and nucleic acid purification. Equipped with the FA-24×2 fixed-angle rotor, it accommodates up to 24 tubes sized 1.5/2.0 mL, delivering a maximum speed of 15,060 rpm and a centrifugal force of up to 21,300 × g.

Key technical features include:

  • High-speed centrifugation essential for rapid pellet formation and phase separations in DNA and RNA extraction protocols.
  • Precise timed runs from 10 seconds to 99:59 hours, plus a continuous operation mode for extended bioprocessing steps.
  • Operator-friendly controls with one-finger lid closure, automated lid opening, and a dedicated short-spin key enable efficient workflow integration.
  • Fast acceleration and deceleration times of 15 seconds each, complemented by 10 selectable soft-brake ramp settings, allow fine control over sample handling, minimizing disturbances to delicate biomolecules.
  • Compact footprint of 10 x 14 x 9 inches and a weight of 45 lbs, ideal for space-limited laboratory environments commonly found in clinical diagnostics and synthetic biology labs.
  • Low noise operation under 56 dB(A) enhances the comfort of prolonged laboratory use.

This microcentrifuge is widely adopted in biotechnology and molecular diagnostics research, supporting critical applications such as gene editing sample preparation, RNA isolation, and biopharmaceutical manufacturing workflows. Its compact design and robust performance make it a reliable instrument essential for bioprocessing and molecular cloning workflows requiring consistent, efficient centrifugation.

The Eppendorf 5420 is powered at 120V 50/60Hz with a maximum power consumption of 270 W, ensuring compatibility with standard laboratory electrical systems. This unit supports numerous biomedical engineering applications including live-cell imaging sample prep and fluorescence analysis, where precise centrifugation parameters critically impact downstream assay reliability.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 5 November 2026