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Thermo PikoReal 96 Real-Time PCR Thermal Cycler with 5 Optical Channels for Multiplexing

From€2,380.37excl. VAT

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Brands
Thermo Fisher Scientific
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Used
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Product description

High-precision Thermo PikoReal 96 thermal cycler enables multiplex qPCR with 5 channels and precise temperature control.

The Thermo PikoReal 96 Real-Time PCR System is a high-precision thermal cycler designed for reliable quantitative PCR (qPCR) and multiplex gene expression analysis. Featuring a superior *thermal block design* with a temperature range of 4 to 99.9°C and an accuracy of ±0.2°C, it supports rapid heating rates exceeding 5°C/sec and cooling at 4.5°C/sec. The system incorporates *five optical channels* spanning excitation wavelengths from 475 to 640 nm and detection from 520 to 740 nm, enabling multiplex amplification of up to 4 target genes simultaneously.

This thermal cycler operates as a standalone unit or via PC-controlled flexible *PikoReal software*, allowing real-time experiment monitoring and USB protocol management. Its compact design dramatically *reduces plastic waste* and reagent consumption, making it a cost-effective solution for molecular diagnostics and bioprocessing workflows. The Ethernet interface supports connecting up to 10 instruments to a single PC, facilitating high-throughput setups critical in synthetic biology and molecular cloning applications.

Key functionalities include absolute and relative quantification modes, high-resolution melt (HRM) analysis, allelic discrimination, and melt curve analysis. The system’s precise temperature control and multiplex capabilities make it essential for advanced biotechnology research, including gene expression profiling, molecular diagnostics, and biopharmaceutical production pipelines.

Specifications:

  • Dimensions: 9 x 12 x 12 inches
  • Sample volume range: 5–20 µL per well (PikoReal 96 block)
  • Dynamic range: 10 logs
  • Scan time: <10 seconds for four multiplexing channels
  • Automatic heated lid temperature and pressure control
  • Weight: 11.97 kg (26.4 lbs)

This unit is manufactured in the USA and verified for full functionality despite minor cosmetic imperfections. It is widely adopted in clinical diagnostics, synthetic biology research, and live-cell fluorescence analysis, offering researchers a precise and efficient platform for qPCR applications.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 7 November 2026