Applied Biosystems StepOne Plus 96-Well Real-Time PCR System with 4-Color Multiplex Detection

The Applied Biosystems StepOne Plus is a high-precision 96-well real-time PCR system designed for quantitative gene expression analysis, molecular diagnostics, and biotechnological research. Featuring a 4-color optical detection system (FAM/SYBR, VIC/JOE, NED/TAMRA, ROX), it enables multiplex assays critical for molecular cloning workflows and synthetic biology research.

Key technical features include:

• Peltier thermal block with VeriFlex technology, delivering six independent temperature zones with ±0.25 °C uniformity to ensure optimal reaction conditions essential in molecular diagnostics and bioprocessing applications.
• Fast cycling mode capable of completing runs in under 40 minutes with a temperature ramp rate up to 2.2 °C/s, as well as a standard cycling mode with up to 1.6 °C/s ramp rate, supporting diverse experimental protocols.
• Standalone operation via touchscreen interface or network control through LAN connection, facilitating flexible integration into biomanufacturing pipelines and clinical diagnostics laboratories.
• Comprehensive StepOne software suite providing tools for relative quantification (ΔΔCt method), standard curve generation, High Resolution Melting (HRM) analysis, and genotyping assays — critical for gene editing validation and molecular diagnostics workflows.
• Low energy consumption design and approximately 24 kg weight for benchtop use in biotechnology and biomedical engineering labs.

This system is widely adopted in synthetic biology research and biotechnological investigations, offering reliable, precise, and multiplexed real-time PCR detection vital for advanced molecular biology and biopharmaceutical production pipelines.