Applied Biosystems 7500 FAST Real-Time PCR System for Fast Multiplex Genetic Analysis

The Applied Biosystems 7500 FAST real-time PCR system is engineered for rapid and precise molecular diagnostics and biotechnology research workflows, including gene expression analysis, pathogen detection, and molecular cloning applications. Its 96-well block format supports sample volumes from 10 to 100 µL, optimized for fast PCR with ramp rates exceeding 2°C/sec, delivering results typically within 30–35 minutes.

Key features include:

• Excitation source: tungsten-halogen lamp enabling robust fluorescence excitation across 5 detection channels for multiplex PCR assays.
• Dynamic range: covers 9 logs, facilitating detection from low to high template concentrations, critical in bioprocessing and molecular quantification.
• Temperature control: 4–99.9 °C range with precision of ±0.25 °C between 35–99.9 °C ensures high reproducibility in amplification reactions.
• Compatibility: supports standard 96-well plates and 0.2 mL PCR tubes, accommodating diverse sample preparation protocols.
• User interface: LCD screen with automatic calibration simplifies system operation and maintenance.
• Connectivity: USB and Ethernet ports for seamless integration with laboratory information management systems (LIMS) and remote monitoring.
• Included software and PC: enables data acquisition, analysis, and reporting, essential for clinical diagnostics labs and synthetic biology research.

With a compact footprint (50 x 27 x 40 cm) and weighing 26 kg, this refurbished device meets the demanding throughput of biopharmaceutical production pipelines and biomedical research laboratories. Its advanced multiplex analysis capabilities make it a valuable tool for researchers in synthetic biology, gene editing, and molecular diagnostics seeking efficient, high-throughput amplification and fluorescence detection.