Qiagen HotStarTaq DNA Polymerase

Name: Qiagen HotStarTaq DNA Polymerase
Brand: Qiagen
SKU: 72694
Availability: PreOrder

UsedQiagenPCR equipmentNetherlands

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Enzyme Concentration: 5 units/µl
5'–>3' Exonuclease Activity: Yes
3'–>5' Exonuclease Activity: No
Self-Priming Activity: No
Contaminating Nucleases: No
Availability: Procurable
Lead time: Confirmed with your quote

All 19 specifications

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Specifications 19

Brands: Qiagen
New/Used: Used
Enzyme Concentration: 5 units/µl
5'–>3' Exonuclease Activity: Yes
3'–>5' Exonuclease Activity: No
Self-Priming Activity: No
Contaminating Nucleases: No
Contaminating RNases: No
Contaminating Proteases: No
Extra A Addition: Yes

Buffer Composition: QIAGEN PCR Buffer with KCl and (NH₄)₂SO₄
Additive: Q-Solution
Extension Rate: 2–4 kb/min at 72°C
Half-life at 97°C: 10 min
Half-life at 94°C: 60 min
Amplification Efficiency: ≥10⁵ fold
Activation Time: 15 min at 95°C
Availability: Procurable
Lead time: Confirmed with your quote

Product description

Heat-activated DNA polymerase ensuring specific PCR amplification with QIAGEN buffer and Q-Solution.

HotStarTaq DNA Polymerase is a heat-activated enzyme designed to deliver highly specific and efficient DNA amplification across various PCR applications.

Chemically mediated hot start: Ensures complete inactivation of the polymerase until the initial heat activation step, preventing nonspecific amplification and primer-dimer formation.
Innovative QIAGEN PCR Buffer: Minimises nonspecific products and primer dimers, enhancing reaction specificity.
Q-Solution additive: Facilitates efficient amplification of challenging templates, such as GC-rich sequences.
Room-temperature setup: Allows convenient reaction assembly without the need for ice or cold rooms.

This enzyme is widely utilised in fields such as molecular biology, genomics, and biotechnology. It is particularly valuable in applications like gene expression analysis, genotyping, and mutation detection. Commonly employed in university laboratories, biomedical research centres, and biotechnology companies, HotStarTaq DNA Polymerase has been a reliable tool for researchers since its introduction.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.