Roche LightCycler 1.5 Real-Time PCR System with LC Carousel 2.0 Centrifuge

The Roche LightCycler 1.5 enables *real-time online PCR* with ultra-rapid cycling and simultaneous fluorescence-based quantification and analysis. It includes melting curve analysis for mutation detection and product characterization.

• Sample capacity: 32 per run
• Sample volume: 20 µl
• Detection type: High Brightness LED with peak wavelength 470 nm
• Optical filters:
• Interference filter bandpass 470 nm, HBW 40 nm
• Detector channels with bandpass filters:
• Channel 1: 530 nm, HBW 20 nm
• Channel 2: 645 nm, HBW 30 nm
• Channel 3: 710 nm, HBW 40 nm
• Detector: Photohybrid with 12-bit resolution
• Acquisition time:
• Single capillary: 20 ms
• 32 capillaries: 5 sec
• Temperature range: 40°C to 98°C
• Temperature accuracy: ±0.4°C
• Heating/cooling rates (20 µl sample):
• Heating 40°C to 95°C: <15 sec
• Heating 50°C to 72°C: <8 sec
• Heating 72°C to 95°C: <8 sec
• Cooling 95°C to 40°C: <24 sec
• Cooling 95°C to 60°C: <7 sec
• Dimensions (H x W x D): 400 x 300 x 450 mm
• Weight: 20 kg

The LC Carousel 2.0 Centrifuge is integrated for sample preparation, complementing the PCR system’s high-throughput capacity.