Beckman Coulter BioLector XT Microfluidic Microbioreactor System for 32-Well High-Throughput Cultivation

The Beckman Coulter BioLector XT Microbioreactor is a high-throughput microfluidic cultivation platform designed for parallel microbial strain screenings and bioprocess development. Supporting a 32-well microtiter plate format, it accommodates culture volumes from 800 µL to 2400 µL with precise microfluidic gassing via the M2P-E-BD32 gassing lid, enabling controlled gas exchange critical in bioprocess scale-down modeling.

Key technical features include:

• Microfluidic module (p/n: M2P-E-MFXT) for automated liquid handling, dosing, and pH control via acid/base addition essential in synthetic biology and pharmaceutical biomanufacturing workflows.
• Integrated optical sensors to monitor biomass, pH (range 5.0–7.5, ±0.1 deviation), and dissolved oxygen (0–100% saturation) with up to 6 configurable optical filters spanning a wavelength range of 365 nm to 800 nm, supporting multi-parameter fluorescence and biomass detection widely used in molecular diagnostics.
• Temperature control from approximately 8 °C below ambient to 50 °C, combined with shaking frequencies adjustable from 100 to 1500 rpm on a 3 mm orbit shaker, suitable for optimizing physiological conditions for bacteria, yeast, and fungal cultures.
• The system enables real-time monitoring and automated fed-batch cultivation using microfluidic feeding profiles, making it critical for early-stage bioprocess optimization, recombinant protein expression, and metabolite screening.

The package includes the BioLector XT microbioreactor unit, valve control unit, a computer with BioLector 5.5.4 software, filter modules, compressed air hose, power supply capable of 100–240 VAC input converting to 24 V DC output, and all necessary communication cables.

All components undergo rigorous validation including sensor calibration, shaker performance at max 1500 rpm, heater functionality to 50 °C, humidity control, vacuum control unit testing, and microfluidic pH protocol verification to guarantee full operational readiness. This ensures reliability and accuracy for biotechnology researchers engaged in gene editing validation and molecular cloning workflows.

The instrument is widely adopted for:

• High-throughput microbial strain screening and media composition optimization
• Automated fed-batch bioprocess development and synthetic biology pathway testing
• Real-time biomass, pH, and dissolved oxygen measurement for fermentation process control
• Screening recombinant strains for protein and metabolite production in biopharmaceutical pipelines

This microbioreactor system is ideal for biotechnology, molecular biology, and clinical diagnostics laboratories requiring precise process control and high-content data acquisition in a compact, automated format.

Keywords: microbioreactor, microfluidic microbioreactor, high-throughput microbial cultivation, real-time biomass monitoring, pH and dissolved oxygen sensors, automated fed-batch bioprocessing, synthetic biology research, molecular diagnostics, bioprocess scale-down, recombinant protein expression, microbial strain screening, biopharmaceutical production.