Beckman Coulter BioLector XT Microbioreactor with Microfluidic Module M2P-G-BLXT

Beckman Coulter BioLector XT Microbioreactor

The Beckman Coulter BioLector XT is a 32-well high-throughput microbioreactor for parallel microbial cultivation, real-time process monitoring, and microfluidic bioprocess development. It is suited to bacteria, yeast, and fungi screening, recombinant protein expression, synthetic biology workflows, and bioprocess scale-down studies.

Included configuration

• BioLector XT microbioreactor, part number M2P-G-BLXT
• Valve control unit, part number M2P-E-MFXT
• Computer with BioLector 5.5.4 Software
• Biomass filter modules
• E-BD32 microfluidic gassing lid for standard microfluidic experiments
• Foil applicator
• 110 VA power supply: 100–240 VAC, 50/60 Hz input, 24 VDC output
• Compressed-air hose
• Small and large access keys for the valve control unit
• Communication and power cables

Technical specifications

• Plate format: 32 wells
• Cultivation well filling volume: 800–2400 µL, dependent on shaking speed and microtiter plate type
• Maximum reservoir well volume: 1800 µL with FlowerPlate; 2000 µL with Round Well Plate
• Temperature range: approximately 8 °C below ambient to 50 °C
• Shaker orbit: 3 mm
• Shaking frequency: 100–1500 rpm
• Filter capacity: up to 6 different filters
• Installed filters: biomass; 3 pH filters (HP8, LG1, ph51); 2 dissolved oxygen filters (Pst3, RF)
• Wavelength range: 365–800 nm
• Microtiter plate read time: approximately 1.8 minutes per parameter per 32 wells, depending on the measured parameter
• pH measurement range: approximately 5.0–7.5, with deviation below 0.1
• Dissolved oxygen measurement range: 0–100% oxygen saturation
• pH control: acid and/or base addition
• Integrated optical measurement of biomass, pH, dissolved oxygen, and fluorescence

Applications

• High-throughput microbial strain screening
• Media and growth-condition optimization
• Automated fed-batch cultivation using microfluidic feeding profiles
• Real-time biomass, pH, dissolved oxygen, and fluorescence monitoring
• Recombinant protein expression and strain development
• Early-stage bioprocess development and scale-down modeling
• Metabolite production screening, including organic acids and enzymes
• Synthetic biology pathway testing and workflow optimization
• Biotechnology, molecular biology, and biopharmaceutical process development

Functional testing

The pre-owned BioLector XT was powered up, initialized, and tested by the service team. Testing included sensor calibration, door and door-lock verification, shaker operation to 1500 rpm, heater operation to 50 °C, manual humidity control, vacuum and pressure performance, and execution of a microfluidic pH protocol without errors.