Sartorius Stedim Biostat B Twin Bioreactor with 2-Liter Autoclavable Glass SW Vessels

The Sartorius Stedim Biostat B Twin Bioreactor is a controlled parallel bioprocess system engineered for rigorous cell culture applications using two independent 2-liter single-wall autoclaveable glass vessels. Ideal for biotechnology research, molecular diagnostics, and bioprocess development, the system enables precise regulation of critical culture parameters including pH, dissolved oxygen (DO), temperature, agitation, and multi-gas mixing.

Key Specifications and Features:

• Bioreactor Configuration: Twin control tower supporting two parallel 2 L single-wall glass vessels with working volumes of 0.4 to 2 L (max volume 3 L).
• Agitation: Direct drive 200 W motor per vessel with variable speed range 20–2000 RPM and a 3-blade pitched impeller for gentle mixing, suitable for shear-sensitive mammalian and insect cells.
• Temperature Control: External heating blankets with cooling finger options, capable of maintaining vessel temperatures from 8 °C above cooling-water inlet up to 60 °C.
• Gas Supply and Mixing: Four-gas mixing system (Air, O₂, N₂, CO₂) with precision thermal mass flow controllers (±1% full scale) and solenoid-controlled rotameters for controlled aeration and sparging, ensuring reproducible culture oxygenation and pH balance.
• Sensors and Inputs: Includes pH and DO optical sensors, temperature sensors (Pt100), Redox sensors compatibility, and ports for gravimetric feed control; integrated exhaust condensers maintain vapor condensation and heat management.
• Peristaltic Pumps: Built-in Watson Marlow fixed-speed pumps for precise acid/base addition and medium feeding, critical for fed-batch bioprocess strategies.
• Control Interface: Touchscreen display with comprehensive digital connectivity (RS-232, M12 plugs) for sensor integration and process monitoring; enables independent or synchronous operation of both culture vessels.
• Physical Dimensions & Power: Footprint of 35×82.2×43 cm (W×D×H) and 55 kg weight; requires 120 V, 60 Hz power with max 12 A current consumption.

Applications:

• Mammalian and insect cell culture under controlled bioprocess conditions
• Seed train development and scale-up studies for biomanufacturing pipelines
• Fed-batch and batch culture process optimization for suspension cells
• Media and feeding strategy evaluation in biotechnology and molecular biology research
• Metabolic kinetics and growth rate studies critical to synthetic biology workflows
• Early-stage biopharmaceutical production and quality control

This bioreactor system is widely adopted among researchers in biotechnology, molecular diagnostics, biomedical engineering, and synthetic biology, providing essential control for live-cell imaging, fluorescence analysis, and small-scale bioprocessing tasks that underpin gene editing and bioprocess development workflows.

Included Components:

• Dual main control tower
• Two direct drive motors with cables
• Two 2-Liter single-wall autoclaveable glass vessels
• Two heating blankets and exhaust condensers
• pH, DO, and temperature sensors with cabling
• Power cord

Note: Tubing and consumables are not included but may be sourced additionally.

The Sartorius Stedim Biostat B Twin Bioreactor undergoes extensive quality verification prior to sale, including calibration against NIST standards, functional testing of pumps and sensors, and system software validation to ensure stable operation in demanding bioprocess and cell culture environments. Shipping is insured, and technical support including remote setup and training is available to facilitate integration into research workflows.

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This system is essential for bioprocess optimization, molecular cloning workflows, and small-scale bioproduction, offering a compact and precise solution for parallel cell culture experiments in academic and industrial biotechnology laboratories.