Sartorius Biostat B Twin Benchtop Bioreactor with Two 10 L Jacketed Autoclavable Glass Vessels

The Sartorius Biostat B Twin is a dual-vessel benchtop bioreactor system designed for controlled microbial cultivation, fermentation process development, and small-scale bioprocess optimization. It includes two 10 L jacketed, autoclavable glass culture vessels with a 13 L total vessel volume.

• Configuration: Twin control tower supporting 2 vessels with independent parallel fermentation runs
• Working volume: 1.5 to 10 L per vessel
• Vessel type: Autoclavable double-wall glass vessels with jacketed temperature control
• Mixing: Rushton impellers and foam disc
• Aeration: Ring sparger
• Agitation: Direct-drive 200 W motor; 20–2000 rpm, with 800 rpm specified for the 10 L double-wall configuration
• Gas control: Air and O₂ supply through 2 thermal mass flow controllers and 2 flow meters
• Gas precision: Thermal mass flow controllers, ±1% full scale; flow meters, ±5% full scale
• Gas supply pressure: Maximum 1.5 barg
• Gas flow: Air up to 20 L/min and O₂ up to 20 L/min through mass flow controller outputs
• Temperature control: Open thermostat system with recirculation pump and automatic cooling-water valve
• Temperature range: 8°C above cooling-water inlet temperature to 80°C
• Heating capacity: 600 W
• Integrated pumps: Four Watson-Marlow 114 fixed-speed pumps for pH control and substrate addition
• Pump speeds: SUBS A, SUBS B, and ACID at 5 rpm; BASE at 44 rpm
• Sensors included: Two pH sensors with cables, two dissolved oxygen sensors with cables, and two temperature sensors
• Feed monitoring: Two EVO1S1-WV4 feed/supply weight scales, 4 kg maximum capacity
• Turbidity: Turbidity-capable controller input; turbidity sensors are sold separately
• Controller connections: RS-232 scale inputs, turbidity input, Pt100 temperature input, pH/redox input, pO₂ input, thermostat interfaces, exhaust cooler controls, sparger aeration control, external signal inputs, and external peristaltic pump connections
• Temperature sensor specification: Pt100, 0–150°C, ±0.1°C accuracy
• Power requirements: 120 V ±10%, 60 Hz, maximum power consumption 12 A
• Dimensions: 35 × 82.2 × 43 cm (14 × 32 × 17 in)
• Net weight: 55 kg (121 lb)

This parallel bioreactor system is suitable for microbial fermentation, strain screening, media and nutrient optimization, batch and fed-batch studies, aeration and agitation strategy testing, and bioprocess development. It supports controlled pH, dissolved oxygen, temperature, agitation, gas mixing, substrate feeding, and oxygen-transfer studies used in biotechnology, synthetic biology, and biopharmaceutical production workflows.