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BioTek ELx405 Automated Microplate Washer for 96- and 384-Well Plates

UsedBioTekImmunohistochemistryUnited States

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Microplate Types
96-well plates/strips 96- and 384 well plates/strips (model with U) Low profile and standard height
Manifold Types
96 Tube Dual Action manifold for 96-well washing or 96-/384-well microplate washing: 96-tube (8 x 12) manifold + Dual-Action (models with U) 96-/384 Deep well or standard well microplates (DW model)
Sterilization
Chemical
Flow Rates
High flow to low flow Optimized rates for cell assays
Wash Speed
96-well: < 30 seconds: 3 aspirate / dispense cycles, 300 µL/well, no soak, final aspirate 384-wells, 100 µL/well, 3 cycles: <80 seconds (Model with U)
All 19 specifications
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Specifications 19

Brands
BioTek
New/Used
Used
Microplate Types
96-well plates/strips 96- and 384 well plates/strips (model with U) Low profile and standard height
Manifold Types
96 Tube Dual Action manifold for 96-well washing or 96-/384-well microplate washing: 96-tube (8 x 12) manifold + Dual-Action (models with U) 96-/384 Deep well or standard well microplates (DW model)
Sterilization
Chemical
Flow Rates
High flow to low flow Optimized rates for cell assays
Wash Speed
96-well: < 30 seconds: 3 aspirate / dispense cycles, 300 µL/well, no soak, final aspirate 384-wells, 100 µL/well, 3 cycles: <80 seconds (Model with U)
Dispense Precision
≤4.0% CV: 300 µL/well (96-well washing)
Residual Volume
<2 µL/well after a 3-cycle wash
Supply bottle
3.7 L
Buffer/reagent Selection
Auto switching module for up to 4 buffers (option)
Wash Cycles
1 -10
Volume Range
25 to 3000 microliter increments
Soaking
1 to 600 seconds
Shaking
User-programmable speeds and timing
Onboard Software
2×24 character LCD display Create, edit or run up to 75 protocols Multiple pre-programmed maintenance protocols
Weight
40 lbs
Dimensions
17x29x15 in
Power
100 to 204V, 50/60Hz, 8.0A

Product description

Automated microplate washer for precise 96/384-well washing with programmable cycles, chemical sterilization, and low residual volume.

The BioTek ELx405 is a versatile and programmable automated microplate washer designed for efficient washing of 96- and 384-well plates and strips, supporting both standard and low profile microplates. Suitable for cell-based and ELISA assays, it features multiple configurations tailored to varying plate formats and manifold requirements.

Key technical specifications include:

  • Programmable wash cycles: 1 to 10 cycles with adjustable soak times (1–600 seconds), user-defined shaking speeds, and configurable flow rates optimized for cell assays.
  • High throughput washing: Typical 96-well protocols complete in under 30 seconds (three aspirate/dispense cycles, 300 µL/well), while compatible 384-well models perform washes in less than 80 seconds (100 µL/well).
  • Precision and low residual volume: Dispense precision rated at ≤4% CV for 300 µL per well in 96-well format, with residual volume <2 µL per well after a three-cycle wash.
  • Manifold options: Includes 96-tube dual-action manifolds and 96-/384-well manifolds (models with U) supporting deep well and standard well plates.
  • Chemical sterilization: Enables integration into sterile ELISA and cell-based assay workflows, enhancing assay reliability in molecular diagnostics and bioprocessing applications.
  • Protocol management: Onboard LCD interface supports creation, editing, and execution of up to 75 custom wash protocols and preprogrammed maintenance routines.
  • Buffer management: Optional auto-switching module accommodates up to 4 different buffers or reagents for complex washing sequences.
  • Physical specs: Dimensions 17×29×15 in, weight 40 lbs, and power operation from 100 to 204V at 50/60Hz.

Widely used in biotechnology, molecular diagnostics, and biomedical research laboratories, the BioTek ELx405 offers reliable microplate washing essential for workflows involving cell imaging, immunoassays, and biopharmaceutical production pipelines. Its adaptability to different plate formats and stringent wash control makes it an indispensable tool in molecular cloning and synthetic biology research environments.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 6 November 2026