Luminex FlexMAP 3D Multiplexing Flow Cytometry Microplate Reader

The Luminex FlexMAP 3D is a flow-based bead reader and multiplexing flow cytometry microplate reader designed for high-throughput analysis of up to 500 analytes per well using xMAP bead-based technology. It supports 96- and 384-well microtiter plates with sample volumes ranging from 10 to 200 µL. Equipped with dual lasers—a 532 nm Reporter Laser and a 638 nm Classification Laser—and sensitive photomultiplier tubes and avalanche photodiodes, FlexMAP 3D provides precise bead classification and signal detection with a dynamic range of 4.5 logarithmic units.

Key technical features include:

• Multiplexing capability for simultaneous detection and quantitation of up to 500 unique xMAP microspheres per sample, essential for multiplex immunoassays and biomarker validation.
• Automated fluidics system with two syringes, sample injection rate of 2 µL/second, and robust flow control ensuring linear and stable sample flow for reproducible data within molecular diagnostics and bioprocessing workflows.
• Compatibility with standard 96- and 384-well microplates, facilitating integration into established molecular cloning and synthetic biology protocols.
• Sample throughput of approximately 20 minutes per 96-well plate and 75 minutes per 384-well plate, suited for high-throughput screening and vaccine response studies.

The instrument includes the Xponent software (version 4.3) for control, data acquisition, and report generation, and features stringent maintenance and calibration protocols to ensure optimal laser alignment, minimal electronic noise, and consistent fluidic performance. It is widely adopted in biotechnology research fields such as gene expression analysis, cytokine profiling, immunoassay development, and biomedical engineering.

Its design supports comprehensive multiplex immunoassay workflows critical in biopharmaceutical production pipelines and molecular diagnostics, offering sensitive, reproducible, and automatable results suitable for clinical research and biomanufacturing applications.

This FlexMAP 3D system is fully serviced, inspected for fluidics and optics integrity, and comes with a warranty and technical support, ensuring reliability for demanding laboratory environments. The system’s advanced multiplexing capability makes it indispensable for researchers focusing on biotechnology and molecular biology, enabling efficient, large-scale assays with minimized cross-reactivity and accurate biomolecule quantification.