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Procurable · sourced on request

Cell Signaling Technology 4060

UsedCell Signaling TechnologyFlow cytometry antibodiesNetherlands
Procurable

Not in stock, available to order through our sourcing team

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Molecular Weight
60 kDa
Storage Temperature
-20°C
Species Reactivity
Human, Mouse, Rat, Bovine, Drosophila, Hamster, Simian, Other
Applications
Western Blotting, Fluorescent Western, Simple Western™, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Dilution Recommendations for Western Blotting
1:2000
Availability
Procurable
Lead time
Confirmed with your quote
All 14 specifications
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Specifications 14

Brands
Cell Signaling Technology
New/Used
Used
Molecular Weight
60 kDa
Storage Temperature
-20°C
Species Reactivity
Human, Mouse, Rat, Bovine, Drosophila, Hamster, Simian, Other
Applications
Western Blotting, Fluorescent Western, Simple Western™, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Dilution Recommendations for Western Blotting
1:2000
Dilution Recommendations for Immunohistochemistry (Paraffin)
1:50 - 1:200
Dilution Recommendations for Immunofluorescence (Immunocytochemistry)
1:400 - 1:800
Dilution Recommendations for Flow Cytometry (Fixed/Permeabilized)
1:100 - 1:400
Dilution Recommendations for Immunoprecipitation
1:50
Dilution Recommendations for Fluorescent Western
1:2000
Availability
Procurable
Lead time
Confirmed with your quote

Product description

Monoclonal antibody detecting Akt phosphorylation at Ser473, validated in multiple immunoassays across species.

The Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb is a monoclonal antibody designed to detect the phosphorylation of Akt at serine 473, a critical modification in the Akt signalling pathway. This antibody is essential for researchers investigating cell survival, proliferation, and metabolism.

Key features:

  • High specificity and sensitivity for phosphorylated Akt (Ser473), ensuring accurate detection in various assays.
  • Validated applications include Western blotting, immunohistochemistry, immunofluorescence, flow cytometry, and immunoprecipitation.
  • Cross-reactivity with multiple species, such as human, mouse, rat, bovine, Drosophila, hamster, and simian, facilitating diverse experimental models.
  • Produced by Cell Signaling Technology, a leader in antibody development, ensuring consistent quality and performance.

This antibody is widely used in biotechnology and biomedical research, particularly in studies related to cancer biology, neuroscience, and metabolic disorders. It is commonly employed in university laboratories, biomedical research centres, and clinical research institutions. First released in 2010, the Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb remains a critical tool in current research, reflecting its enduring relevance and reliability.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 5 November 2026