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Procurable · sourced on request

Cell Signaling Technology 9271

UsedCell Signaling TechnologyFlow cytometry antibodiesNetherlands
Procurable

Not in stock, available to order through our sourcing team

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Molecular Weight
60 kDa
Sensitivity
Endogenous
Source
Rabbit
Storage Conditions
–20°C
Storage Buffer
10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol
Availability
Procurable
Lead time
Confirmed with your quote
All 14 specifications
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Specifications 14

Brands
Cell Signaling Technology
New/Used
Used
Molecular Weight
60 kDa
Sensitivity
Endogenous
Source
Rabbit
Storage Conditions
–20°C
Storage Buffer
10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol
Reactivity
Human, Mouse, Rat, Hamster, Monkey, D. melanogaster, Bovine, Dog
UniProt ID
P31751, Q9Y243, P31749
Entrez-Gene ID
208, 10000, 207
Applications
Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry
Dilution Recommendations
Western Blotting: 1:1000; Immunoprecipitation: 1:100; Immunofluorescence (Immunocytochemistry): 1:50 - 1:200; Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400
Availability
Procurable
Lead time
Confirmed with your quote

Product description

Polyclonal antibody detecting Akt phosphorylation at Ser473 across multiple species for molecular research.

The Phospho-Akt (Ser473) Antibody is a polyclonal antibody developed by Cell Signaling Technology to detect the phosphorylation of Akt at serine 473. This antibody is instrumental in studying the PI3K/Akt signalling pathway, which is crucial for regulating cell growth, survival, and metabolism.

Key features include:

  • High specificity: Recognises Akt phosphorylated at Ser473 without cross-reacting with non-phosphorylated forms.
  • Versatile applications: Validated for use in Western blotting, immunocytochemistry, immunoprecipitation, and flow cytometry.
  • Broad reactivity: Reacts with human, mouse, rat, bovine, canine, Drosophila, hamster, and porcine samples.
  • Reliable performance: Consistently cited in peer-reviewed research for its role in detecting Akt phosphorylation.

This antibody is widely utilised in molecular biology, cancer research, and biotechnology to investigate Akt activation and its implications in various cellular processes. It is commonly employed in university laboratories, biomedical research centres, and biopharmaceutical companies focusing on signal transduction and therapeutic target validation.

First released prior to 2010, the Phospho-Akt (Ser473) Antibody remains a critical tool in contemporary research, reflecting Cell Signaling Technology's commitment to providing high-quality reagents for the scientific community.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 15 November 2026