Waters XBridge BEH C18 3 mm × 50 mm HPLC Column, 3.5 µm, 130Å Pore Size

The Waters XBridge BEH C18 Column (P/N 186003026) is an analytical HPLC column engineered for high-performance reversed-phase chromatography across a broad pH range (1–12), ideal for complex molecular separations in biotechnology, molecular diagnostics, and pharmaceutical analysis. This column features:

• Trifunctionally bonded C18 ligand ensuring universal applicability for most HPLC separations
• Hybrid particle substrate (BEH technology) with a spherical particle shape and 3.5 µm particle size, optimizing efficiency and resolution
• Pore size of 130Å suitable for a wide range of biomolecules including peptides and small proteins
• Low silanol activity and endcapping providing superior low pH stability and ultra-low column bleed, critical for sensitive detection methods
• Dimensions: 3 mm internal diameter × 50 mm length, fitting benchtop HPLC systems commonly used in molecular biology and bioprocessing workflows
• Surface area of 185 m²/g and 18% carbon load tailored for high retention and selectivity in reversed-phase mode
• Classified as USP L1, meeting stringent regulatory standards for pharmaceutical and clinical research applications

This column is extensively utilized in biotechnology research pipelines, including gene editing and synthetic biology experiments that demand reliable and reproducible chromatographic performance. Its chemically robust design makes it a versatile tool for biopharmaceutical production pipelines and molecular cloning workflows, where stable chromatographic separation under variable pH is essential. The product is supplied brand new, sealed, and ready for immediate integration into HPLC systems for advanced analytical applications.