58k+ listingsShips to NLResponse within one hour+31 73 711 4717info@questpair.com

Higgins PROTO300 C18 Semi-Prep Reversed-Phase HPLC Column 25 cm × 20 mm × 10µm

Price on request

In stock

Can't find what you're looking for?

Year
2017
MPN
RS-2520-W181
Condition Description
IN GOOD CONDITION
Weight (lbs)
1
Country/region of manufacture
United States
All 9 specifications
  • Ships to NL
  • Response within 1 hour
  • Warranty available. Buy without worry
  • Support from a scientist

Specifications 9

Brands
Higgins Analytical
New/Used
Used
Year
2017
MPN
RS-2520-W181
Condition Description
IN GOOD CONDITION
Dimensions
12"L x 2"W x 2"H
Weight (lbs)
1
Country/region of manufacture
United States
Custom Label
G4

Product description

High-capacity PROTO300 C18 semi-prep LC column for protein and peptide purification, robust at pH 1.5–10.

The Higgins Analytical PROTO300 C18 Semi-Prep LC Column is engineered expressly for reversed-phase high-performance liquid chromatography (RP-HPLC) applications involving purification of large biomolecules such as proteins and peptides. Specifically designed for bioprocessing workflows, it provides enhanced resolution and high capacity needed in molecular diagnostics and biopharmaceutical production pipelines.

Key technical specifications include:

  • Column dimensions: 25 cm length × 20 mm internal diameter × 10 µm particle size
  • Pore size: 300Å, optimal for large biomolecule separation including cyclized peptides
  • Surface area: 100 m²/g, contributing to high binding capacity
  • Pore volume: 0.9 mL/g, facilitating efficient mass transfer
  • Phase type: Monofunctional & fully endcapped C18 phase enhancing peak shape and reproducibility
  • Resilient under extreme operating pH conditions ranging from 1.5 to 10, accommodating typical bioprocessing column washing protocols without compromising column longevity

This semi-preparative LC column is widely adopted in synthetic biology research and molecular cloning workflows requiring reliable and robust chromatographic separation of peptides and proteins. Its design supports live-cell imaging sample preparation through precise protein purification and is critical in biomanufacturing quality control operations.

Ideal for researchers and laboratory professionals engaged in cell imaging, biotechnology, and clinical diagnostics, the Higgins PROTO300 delivers superior performance for preparative and semi-preparative purification tasks in cutting-edge life sciences research.

Why Questpair exists

Lab equipment markets are fragmented. Buyers spend weeks calling distributors, comparing quotes, and verifying supplier reliability. Sellers struggle to reach global research labs.

We built Questpair to fix this. Every supplier on the platform is verified before listing. Every quote you receive comes from a real, vetted seller. AI prepares your case in minutes, then a real specialist takes over.

We're an independent team based in The Netherlands, working with research labs and procurement teams across Europe and worldwide.

Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Yes. Every verified supplier states which service partners cover the instrument, and we list that alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

Reply in one hour

Can't find the exact configuration?

Tell us what you need and a scientist finds it for you, usually within the hour.

Last updated 19 October 2026