Cytiva Akta Avant 25 Automated Liquid Chromatography System for Protein Purification

The Cytiva Akta Avant 25 Chromatography System is a highly modular and automated liquid chromatography platform designed for precise biomolecular separation and purification workflows. Widely used in protein purification, bioprocess development, and preparative chromatography applications essential for molecular diagnostics and biomanufacturing, this system integrates multiple valve clusters for flexible fluid routing and supports complex method development including gradient formation.

Key technical features include:

• Multiple installed valve modules (sample inlet valves, versatile valves, quaternary, injection, column, pH, and outlet valves) enabling flexible multi-buffer handling and sample routing.
• Dual system pumps and a dedicated sample pump ensure controlled, scalable, and reproducible flow rates critical for consistent chromatographic separations.
• Integrated dynamic mixer for precise gradient generation, enhancing resolution and reproducibility in protein purification workflows.
• Built-in UV monitor, conductivity monitor, and pressure sensors provide continuous real-time monitoring of process parameters to maintain chromatographic integrity.
• Automated fraction collector with cassette trays for high-throughput sample collection supporting downstream analysis or bioprocess scaling.
• Sample loop volume: 2 mL, suitable for preparative scale biomolecule isolation.

The system is commonly employed in:

• Antibody and enzyme purification within biotechnology and pharmaceutical production pipelines.
• Buffer screening and optimization to enhance method development efficiency.
• Process scale-up studies supporting biopharmaceutical manufacturing transitions from R&D to commercial production.
• Quality control and analytical verification within molecular biology and synthetic biology research environments.

Supplied with a computer running Unicorn software v7.8, it supports user-friendly operation and method customization, critical for biotechnology labs focusing on gene editing workflows, synthetic biology experiments, and live-cell imaging sample preparations requiring high-purity biomolecules.

This model is fully tested for:

• Pump priming and flow rate accuracy
• UV, conductivity, and pH sensor performance
• Fraction collector dispensing and rack code identification
• System gradient formation and air sensor functionality

The Akta Avant 25 system is a critical instrument for labs demanding reliable, automated chromatography solutions optimizing molecular cloning workflows and bioprocessing operations. Its flexible design and robust monitoring capabilities make it a preferred choice for research institutions, clinical diagnostics laboratories, and biomanufacturing facilities worldwide.