Amersham Bioscience Äkta Purifier Protein Chromatography System with UV Monitoring and Fraction Collection

The Amersham Bioscience Äkta Purifier System is a modular protein purification and liquid chromatography instrument designed for reliable, high-resolution separations of proteins and peptides. Essential for molecular biology workflows and bioprocessing applications, it supports customizable configurations suitable for biotechnology and molecular diagnostics research.

Key features include:

• Pump P-900 enabling precise flow rates from 0.001 to 10 ml/min for optimal separation control
• UV-900 Monitor with up to 3-wavelength detection and a 10 mm flow cell path length, allowing sensitive multi-wavelength analysis critical for fluorescence and absorbance monitoring in protein purification
• Frac-950 Fraction Collector improving sample collection accuracy during multi-step chromatography processes
• pH/C-900 Monitor providing continuous online pH measurement across a broad range (2–12) with long-term stability (pH 1–13) suitable for maintaining buffer conditions in biomolecular separations
• Integrated motor valves (PV-908 and INV-907) supporting high-pressure operations up to 25 MPa, critical for biomanufacturing and synthetic biology purification pipelines
• Mixer M-925 and Box-900 units enabling effective online buffer selection and media screening for enhanced protein binding and elution profiles

The system’s modular architecture and buffer handling capabilities make it widely adopted in synthetic biology, gene editing, and biopharmaceutical production pipelines where precise protein purification and peak collection are critical. With a compact footprint (450 x 610 x 470 mm) and 38 kg weight, the Äkta Purifier facilitates seamless integration into laboratory environments focused on biotechnology and clinical diagnostics. This instrumentation is ideal for advanced chromatography workflows used extensively in biomedical engineering, molecular cloning, and fluorescence-based protein characterization.