Advion Interchim PuriFlash 5.250 Preparative Flash Chromatography System with UV Detection and Automated Fraction Collector

The Advion Interchim PuriFlash 5.250 is a high-capacity preparative flash chromatography system designed for scalable purification workflows in synthetic chemistry, medicinal chemistry, and bioprocessing. Key features include:

• Flow rate range: 1 to 250 mL/min with <0.25% repeatability and linearity between 0.98 and 1.00
• Maximum operational pressure: 250 bar (3625 psi), supporting large flash column formats for preparative and pilot-scale separations
• UV detection: Diode array detector (DAD) with four selectable wavelengths covering 200–400 nm for real-time monitoring of elution profiles in synthetic organic compound purification and molecular diagnostics
• Injection system: Supports automatic solid and liquid sample injection via a 10-port electric valve, suitable for high-throughput biomanufacturing and synthetic biology workflows
• Automated fraction collection: Equipped with a fraction collector capacity for 3 racks (44 tubes x 25 mL each), enabling reproducible collection integral to biopharmaceutical production pipelines
• Accessories: Includes two dry-load holders, two column holders, solvent tray with drainage system, and communication/power cables for seamless integration
• Dimensions and weight: Total weight approximately 54 kg for user convenience and lab bench compatibility
• Control software: InterSoft X software for precise system management and data acquisition
• Customization: Optional collection racks and preparative C18 columns optimized for biotech and pharmaceutical applications

This system is widely used for preparative purification of synthetic organic compounds and scale-up separations in medicinal chemistry, making it an essential instrument for molecular cloning workflows and synthetic biology research. The robust, industrial-grade construction ensures reliable operation in clinical diagnostics and biotechnology labs focusing on fluorescence analysis and bioprocessing.

The PuriFlash 5.250 supports automated workflows essential for biomanufacturing and offers scalability critical for pilot plant and research environments. High sensitivity UV monitoring combined with precise gradient control enhances separation efficiency in complex reaction mixtures.

Keywords incorporated: preparative flash chromatography system, UV detection, automated fraction collection, synthetic organic compound purification, scalable purification workflow, molecular diagnostics, bioprocessing, synthetic biology research, medicinal chemistry scale-up, biopharmaceutical production pipelines.