Knauer Smartline 5000 HPLC System with UV Detector 2600, Pump 1000, Jetstream 2 Plus Thermostat, and Spark Triathlon 900 Autosampler

The Knauer HPLC System comprises multiple modules optimized for precise chromatographic analysis:

• Smartline Manager 5000:
• Independent channel control (1-4)
• Flow path degassing via fluoropolymer membrane, max flow 10 ml/min
• Degassing capacity approx. 2 ppm oxygen at 1 ml/min
• Pressure rating: 70 PSIG, dead volume ~480 µl per channel
• Materials contacting solvents: PEEK, glass-filled PTFE, Teflon AF
• Suitable solvents: universal except hydrochloric acid and halogenated carbohydrates (e.g., HFIP)

• Smartline UV Detector 2600:
• Lamp types: Deuterium (190–510 nm) and Tungsten Halogen (430–710 nm)
• Detection: 256 diode array with 1.25 nm dot pitch
• Bandwidth selectable: 4–25 nm
• 4 acquisition channels and 4 spectra stored
• Wavelength accuracy ≤1 nm, precision ≤0.5 nm
• Automatic wavelength validation with holmium oxide filter
• Noise (ASTM E1657-94) ≤1×10⁻⁵ AU (D2 lamp), drift ≤5×10⁻⁴ AU/h
• Measurement range up to 2.2 AU, time constants selectable 0.1 to 10 s

• Smartline Pump 1000:
• Double-piston pump with main and auxiliary pistons, automatic backflushing
• Flow rate: 0.001–9.999 ml/min externally controlled
• Flow accuracy <0.5%, precision <0.1% at 1 ml/min and 12 MPa
• Residual pulsation <0.5% at 1 ml/min methanol:water (8:2)
• Max operating pressure 40 MPa
• Expandable with Smartline Manager 5000 to low pressure gradient system (up to 4 eluents)
• Option for high pressure gradient system (HPG)

• Spark Triathlon 900 Autosampler:
• Injection reproducibility: full loop RSD ≤0.3%, partial loop RSD ≤0.5%, µl pick-up RSD ≤1.0%
• Sample capacity: 96 vials (1.5 ml), vial height 32–47 mm inclusive cap
• Injection volume range: 1 µl to 1 ml, increments of 1 µl
• Programmable needle wash and wash solvent bottle (250 ml)
• Built-in vial sensor and compressor for headspace pressure
• Fast sample access (<10 seconds), fast injection valve switching (<100 ms)
• Programmable priority sample option

• Jetstream 2 Plus Column Thermostat:
• Provides precise temperature control for column stability (specific specs not detailed)

This integrated system enables precise separation, detection, and sample handling with advanced degassing, flow accuracy, and UV detection capabilities suitable for routine and advanced HPLC applications.