Waters ACQUITY UPLC H-Class PLUS Bio System with PDA and FLR Detectors for Bioseparations (2021)

The Waters ACQUITY UPLC H-Class PLUS Bio System (2021 model) is a high-performance ultra-performance liquid chromatography (UPLC) platform designed for robust and reproducible bioseparations essential in biotechnology, molecular diagnostics, and biopharmaceutical production pipelines. This system integrates a BioQuaternary Solvent Manager, a BioSample Manager with temperature control, and dual detection technologies: a Photodiode Array (PDA) detector and a Fluorescence (FLR) detector. The configuration supports quaternary solvent blending with automated pH and modifier adjustments, enabling complex gradient formations critical for high-resolution biomolecule characterization.

Key components and features include:

• BioQuaternary Solvent Manager (ACQ-QSM):
• Mixing up to four solvents with vacuum degassing and Auto-Blend Plus™ for online pH, ionic strength, and organic modifier control.
• Low-pressure mixing with 11 precision gradient curves (linear, step, concave, convex).
• Supports expanded solvent choice with optional six-port valve, enhancing flexibility in bioprocessing workflows.

• BioSample Manager-FTN (ACQ-FTN-H):
• Injection volumes: 0.1–10 µL standard, extendable to 1,000 µL with additional loops.
• High accuracy ±0.2 µL and linearity ≥0.999 ensures precise sample introduction.
• Temperature control from 4 to 40 °C with ±0.5 °C accuracy, stabilizing biomolecule integrity during analysis.
• Handles up to 768 samples with various plate formats, supporting high-throughput biomolecular assays.

• PDA Detector (ACQ-PDA):
• Wavelength range 190–500 nm, with ±1 nm accuracy and 1.2 nm optical resolution.
• 500 nL analytical flow cell rated to 1000 psi enables sensitive UV-Vis detection for peptide mapping and protein profiling.

• FLR Detector (ACQ-FLR):
• Excitation/emission spanning 200–890 nm / 210–900 nm suited for fluorescence-based bioassays.
• High sensitivity (S/N>1000) and fast sampling (up to 80 Hz) facilitate detection of labeled biomolecules with low detection limits.
• Flow cell pressure limit at 500 psi ensures robustness.

• Column Manager (CM-A):
• Supports up to four columns with independent temperature zones (4–90 °C), critical for glycan and protein heterogeneity studies.
• Programmable switching valves for automated column use, optimizing workflow efficiency in bioprocessing and analytical development.

This system features biocompatible inert flow paths reducing sample adsorption and degradation, enhancing data reliability for sensitive analytes in synthetic biology, gene editing, and molecular cloning workflows. The integrated software Empower facilitates method development, data acquisition, and workflow integration, making it suitable for academic, clinical, and biopharmaceutical research environments.

Applications of this sensitive and versatile UHPLC system include:

• Characterization and purity testing of monoclonal antibodies
• Glycan profiling and protein heterogeneity analysis
• Peptide mapping and structural confirmation of biomolecules
• Aggregation and stability testing of biologics
• Fluorescence-based detection for labeled biomolecule assays

The system has been fully tested and serviced to ensure operational stability, accurate gradient formation, solvent delivery stability, and precise detector performance. It includes warranty coverage and after-sales technical support. This pre-owned instrument represents a cost-effective, reliable solution for advanced bioseparation workflows critical in biomanufacturing and molecular diagnostics domains.

Keywords naturally integrated: ultra-performance liquid chromatography system, bioseparations, PDA detector, fluorescence detector, biocompatible UHPLC, quaternary solvent mixing, biomolecule analysis, monoclonal antibody characterization, glycan profiling, peptide mapping, molecular diagnostics, synthetic biology research, bioprocessing workflows, biopharmaceutical production.

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This technical description facilitates search visibility among research professionals and institutional buyers seeking robust bio-UPLC systems optimized for biomolecular and biotechnological applications.