Thermo Scientific Vanquish Flex Quaternary UHPLC-MS System with ISQ EC Mass Spectrometer & VWD Detector

The Thermo Scientific Vanquish Flex Quaternary UHPLC-MS System combines state-of-the-art ultra-high performance liquid chromatography (UHPLC) with advanced mass spectrometry (MS) and ultraviolet detection for robust analytical workflows critical in pharmaceutical, environmental, and biochemical research. This integrated platform offers precise solvent delivery, sensitive compound detection, and versatile chromatographic control essential for molecular diagnostics, bioprocessing, and synthetic biology applications.

Key components and specifications include:

• Vanquish Quaternary UHPLC System:
• *Quaternary Pump F*: Serial dual-piston pump with flow range 0.001–8 mL/min, ±0.1% flow accuracy, 2–103 MPa pressure range, and built-in 4-channel solvent degassing for stable gradient formation critical in biomanufacturing process development.
• *Split Sampler FT*: Split loop injection with injection volume 0.01–25 µL, ±0.5% accuracy (10 µL), sample cooling between 4–40°C with ±1°C stability, enabling reproducible injections for live-cell assay sample preparation and molecular cloning workflows.
• *Column Compartment H with Active Preheater*: Temperature range 5–120°C with ±0.05 K stability, dual column slots, and rapid heating/cooling for consistent chromatographic separations in complex biotechnology sample matrices.

• Vanquish Variable Wavelength Detector (VWD) F:
• UV/VIS/NIR photometer with tunable Czerny-Turner monochromator, 190–900 nm wavelength range, spectral bandwidth ~6 nm, wavelength accuracy ±1 nm.
• Flow cell standard volume 11 µL, pressure up to 120 bar (SST), supporting precise absorbance measurements compatible with fluorescence and UV detection assays widely used in clinical diagnostics and cell imaging.

• ISQ EC Mass Spectrometer:
• Electrospray ionization (ESI) source with heated ESI (HESI), mass range m/z 10–1250, high-sensitivity full scan and selected ion monitoring (SIM) modes.
• Scan rate up to 20,000 Da/s, mass accuracy ≤ ±0.1 Da stable over 48 hours, polarity switching within 25 ms for rapid multiplexed detection crucial for metabolomics, proteomics, and gene editing studies.
• Detector DynaMax XR with dynamic range ≥10⁷ supporting trace-level detection essential in molecular diagnostics and biopharmaceutical quality control.

Additional features:

• Integrated Chromeleon software v7.2.10 for instrument control and data analysis with USB license dongle.
• Edwards external vacuum pump included for stable MS operation.
• Comprehensive tested assembly including solvent racks, communication and power cables.
• Fully validated and calibrated, with detailed service and warranty coverage ensuring reliable deployment in critical research environments.

This UHPLC-MS system is extensively used in LC-MS method development, routine quality control, and advanced small molecule impurity profiling in pharmaceutical and biotechnology research. Its precise solvent delivery and temperature control make it a vital instrument in bioprocessing pipelines and synthetic biology experimentation, delivering reproducible, high-sensitivity data necessary for reliable molecular characterization.

Keywords embedded naturally: quaternary UHPLC system, ESI mass spectrometer, variable wavelength detector, HPLC split sampler, molecular diagnostics instrumentation, bioprocessing analytical system, synthetic biology research tool, pharmaceutical impurity analysis, LC-MS method development, biotechnology workflow equipment.

This offering presents a fully functional, serviced, and currently supported configuration, ideal for advanced analytical chemistry, neuroscience, cell imaging, and biomedical engineering laboratories focusing on cutting-edge research and development.