Dionex UltiMate 3000 UHPLC System with Nano Pumps, Autosamplers & VWD Detector

The Dionex UltiMate 3000 UHPLC System is a high-precision ultra high performance liquid chromatography system designed for advanced molecular separation and analysis, widely adopted in biotechnology, pharmaceutical analysis, and environmental monitoring. This versatile UHPLC platform supports bioprocessing workflows and is essential for molecular diagnostics and synthetic biology research requiring ultra-low flow rates and rapid injection cycles.

Key technical components include:

• Autosamplers:
• *WPS-3000TFC*: Operates up to 62 MPa, injection volume precision better than 0.25% for 5 µL full loop injections, cycle times under 60 seconds, ideal for reproducible sample delivery.
• *WPS-3000TPL RS* (Rapid Separation): Supports up to 90 MPa with injection precision <0.4% for 1 µL injections and rapid cycles under 30 seconds, suited for accelerated molecular screening tasks.

• Pumps:
• Two RSL Cnano Pumps (NCP-3200RS) provide ultra-low nanoliter flow rates from 20 nL/min to 50 nL/min at pressures up to 800 bar, enabling high-sensitivity separations crucial in biomanufacturing and gene editing studies requiring precise solvent gradients.
• Integrated ternary micro pumps facilitate sample pre-concentration and multidimensional LC workflows.

• Detector:
• *VWD-3100 Variable Wavelength Detector* covers 190 nm to 900 nm, spanning ultraviolet to visible spectra, with a maximum data rate of 100 Hz, essential for fluorescence analysis and absorbance monitoring in complex biological sample matrices.

• Column Compartment:
• *TCC-3000 RS Rapid Separation Column Compartment* supports column temperatures up to 110°C with post-column cooling to minimize baseline drift, noise, and sample degradation, enhancing reproducibility in biomedical engineering and bioprocess analytics.

• Solvent Racks:
• Two racks each accommodate reservoirs of multiple volumes (five 5L, four 2.5L, and sixteen 0.5L per rack) supporting extended run times and complex solvent gradient configurations.

This UHPLC system is engineered for high-throughput biotechnology laboratories, facilitating critical workflows in molecular cloning, biopharmaceutical production pipelines, and clinical diagnostics requiring high precision, reliability, and rapid sample throughput. Note that software and PC are not included with this system.

Optimized for biotech research applications including live-cell imaging, fluorescence analysis, and bioprocess monitoring, the Dionex UltiMate 3000 UHPLC system offers extensive configurability and robust performance for demanding analytical tasks.