Agilent 1200/6410 Triple Quadrupole LC/MS System for Precise Molecular Diagnostics

The Agilent 1200/6410 Triple Quad LC/MS System integrates high-performance liquid chromatography and triple quadrupole mass spectrometry optimized for molecular diagnostics, biopharmaceutical production, and synthetic biology applications. This system combines multiple precise modules for reliable and reproducible analysis in biotechnology and clinical research workflows.

Key components include:

• Agilent 1200 G1379B Degasser with 4 solvent channels, max flow 5 mL/min, solvent-contact materials PTFE, FEP, PEEK, compatible with pH 1–14, crucial for preventing bubble formation in bioprocessing sample preparation.
• G1312B Binary Pump SL providing precise binary gradient mixing with flow range 0.001–5 mL/min, accurate flow control (±1% or 10 µL/min), operating at pressures up to 600 bar, suitable for high-pressure liquid chromatography in synthetic biology and gene editing assays.
• G1367B High-Performance Autosampler allowing injection volumes 0.1–100 µL with precision <0.25% RSD, high pressure tolerance (400 bar), optimizing sample throughput in molecular biology and biomanufacturing labs.
• G1316A Thermostatted Column Compartment with temperature control from 10°C below ambient to 80°C ensures stable chromatographic separations, supporting temperature-sensitive biomolecule analysis.
• 6410A Triple Quadrupole LC/MS mass spectrometer features:
• MS/MS sensitivity with S/N >20:1
• Mass resolution selectable (0.7 to 2.5 u FWHM)
• Dynamic range >6.5 million, mass range 15–1650 m/z
• Fast scan rate up to 5,200 u/sec; advanced scan modes including MRM, precursor ion scanning critical for targeted proteomics and synthetic biology metabolic profiling
• Automated autotune and solvent declustering for improved ion optics and reproducible results
• High-pressure hexapole collision cell, no detectable cross-talk
• Edwards E2M30 vacuum pump, essential for maintaining required vacuum conditions in MS for stable ion transmission.

The system supports a single-point control data system managing all HPLC and MS functions, streamlining workflows in molecular cloning, bioprocessing, and clinical diagnostics. Its robust design facilitates extensive gradient capabilities with minimal carryover, precise compound quantitation, and reproducible results essential in biopharmaceutical production pipelines and biotechnology research.

Dimensionally, it measures 470 mm (H) x 1110 mm (W) x 660 mm (D) and weighs 108 kg, optimized for benchtop laboratory environments specializing in fluorescence analysis, metabolomics, and targeted molecular diagnostics.

This Agilent 1200/6410 LC/MS system is widely adopted in synthetic biology research, molecular diagnostics, and neuroimaging applications requiring high sensitivity and specificity in compound identification and quantitation.