Agilent 1200 Series High-Performance Liquid Chromatography System with Multi-Wavelength Detector and Binary Pump

The Agilent 1200 Series HPLC System is a modular, high-performance liquid chromatography instrument widely adopted in biotechnology research and molecular diagnostics for precise separation and analysis of complex biological samples.

Key technical features include:

• G1312A Binary Pump: Enables gradient elution with a flow range of 0.05–5 mL/min for controlled solvent delivery essential in bioprocessing and analytical workflows.
• G1329A Autosampler: Provides highly accurate sample injection with injection precision <0.5% RSD, critical for reproducible molecular cloning and synthetic biology experiments.
• G1316A Thermostatted Column Compartment: Maintains stable temperatures from 10 °C below ambient up to 80 °C, supporting consistent chromatographic performance for temperature-sensitive biomolecules.
• G1365D Multi-Wavelength Detector: Offers flexible ultraviolet-visible (UV-Vis) detection with an 80 Hz scanning rate, enabling comprehensive spectral analysis across multiple wavelengths for fluorescence and absorbance assays.
• G1379B Solvent Degasser: Enhances mobile phase preparation by efficiently removing dissolved gases, reducing baseline noise and improving chromatogram stability in clinical diagnostics and biochemical research setups.

This system's compact design ensures space-efficient laboratory configuration. The modular architecture allows customization to specific application needs, making it ideal for cell imaging studies, biomanufacturing quality control, and pharmaceutical analytics. The included solvent rack supports effective mobile phase management.

Specifications:

• Flow range: 0.05–5 mL/min (binary pump)
• Temperature range: Ambient -10 °C to 80 °C (column compartment)
• Detector scan rate: 80 Hz (multi-wavelength detector)
• Injection precision: <0.5% RSD
• Weight: 81.65 kg (180 lbs)
• Shipping dimensions: 54.86 cm x 48.77 cm x 100.58 cm

This preowned HPLC system is verified with power-on functionality and includes user manuals and software, though recalibration and validation are recommended for precise quantitative analysis. It plays a critical role in biotechnological workflows including molecular diagnostics, synthetic biology, and biopharmaceutical production pipelines, providing reliable chromatographic separation and spectral detection needed for advanced research and clinical applications.