Agilent Zorbax Rx-C18 15 cm × 4.6 mm × 5µm Reversed-Phase HPLC Column for Protein and Peptide Separation

The Agilent Zorbax Rx-C18 is a high-performance reversed-phase HPLC column designed for reliable separation of proteins, peptides, and large biomolecules. Key technical specifications include:

• Dimensions: 15 cm length × 4.6 mm inner diameter
• Particle Size: 5 µm, with 300Å pore size enabling efficient entry and separation of large molecules including proteins and polypeptides
• StableBond Technology: Offers superior chemical stability at low pH conditions (e.g., TFA-containing mobile phases), critical for LC/MS workflows
• Wide Compatibility: Optimized for use with acidic mobile phase modifiers such as trifluoroacetic acid (TFA), formic acid, and acetic acid
• Temperature Tolerance: Can operate up to 80°C to enhance sample recovery and resolve difficult proteins more efficiently
• Bonded Phases Availability: The 300Å StableBond family offers multiple bonded phases (C18, C8, C3, CN) to tailor selectivity and recovery for diverse biomolecular applications

This column is widely used in molecular diagnostics, bioprocessing, and biopharmaceutical production pipelines due to its reproducible performance in protein and peptide separation workflows. The wide pore size and stable chemical bonding make it an essential tool for biotechnology research, synthetic biology, and proteomics studies requiring reliable chromatographic resolution and LC/MS compatibility.

Its robustness under acidic conditions and elevated temperatures makes it ideal for biomedical engineering labs, clinical diagnostics, and gene editing research applications where precise biomolecule characterization is required. The Agilent Zorbax Rx-C18 supports advanced biopolymer analysis, synthetic biology research, and biomanufacturing quality control workflows, ensuring consistent and reproducible data critical for scientific and industrial biotechnology environments.