Olympus IX70 Inverted Fluorescence Phase Contrast Microscope with New Filter Sets

Olympus IX70 inverted fluorescence microscope configured for fluorescence, phase contrast, and brightfield microscopy. This pre-owned research microscope is suitable for cell biology, live-cell imaging, neuroscience, immunology, pharmacology, biotechnology, and molecular biology workflows.

Optical and mechanical configuration

• Olympus IX70 inverted microscope base with side camera port
• Mechanical Z-axis
• Binocular head with two eyepieces
• Olympus fluorescence light path
• Mechanical four-position fluorescence filter cube turret
• Mechanical six-position objective turret
• Mechanical XY stage
• Mechanical condenser with PhL and Ph1 phase rings
• Illumination tower
• Camera port configurations: 100% eyepiece/0% camera, 20% eyepiece/80% camera, or 0% eyepiece/100% camera
• 1x C-mount camera adapter

Illumination

• Olympus 100 W HBO fluorescence light source
• Olympus 100 W HBO fluorescence power supply
• Olympus 100 W halogen brightfield light source
• Brightfield power supply integrated into the microscope base
• Reflected fluorescence and transmitted brightfield illumination paths

Objectives included

• 4x/0.13 UPlanFL PhL objective
• 10x/0.30 UPlanFL Ph1 objective
• 20x/0.50 UPlanFL objective

Fluorescence filter sets

The configuration includes three new fluorescence filter sets, selected from the following options:

• DAPI: excitation 365/28 nm, dichroic mirror 405 nm, emission 445/50 nm; compatible with DAPI, Hoechst, and AlexaFluor 350
• EGFP/FITC/Cy2: excitation 475/30 nm, dichroic mirror 500 nm, emission 530/40 nm; compatible with EGFP, FITC, Cy2, and AlexaFluor 488
• TRITC/Cy3/DsRed: excitation 545/20 nm, dichroic mirror 562 nm, emission 605/60 nm; compatible with TRITC, Cy3, RFP, Rhodamine, and AlexaFluor 546
• Texas Red: excitation 560/20 nm, dichroic mirror 585 nm, emission 630/40 nm; compatible with Texas Red, mCherry, and AlexaFluor 594
• Cy5: excitation 630/50 nm, dichroic mirror 662 nm, emission 695/50 nm; compatible with Cy5, AlexaFluor 647, and Draq5. This set may require an upgraded light source.

Imaging system

• Bestscope 8.3MP color CMOS camera
• Dell laptop computer with Windows 10
• ImageJ analysis software
• Camera software
• Image capture for still images and live video
• Raster image display, annotation, editing, calibration, measurement, analysis, processing, printing, and saving
• Variable linear measurement tools, including line segments and radius measurements
• Necessary power and communication cables

Applications

The Olympus IX70 is an inverted fluorescence microscope for fluorescent labeling, phase contrast observation of transparent specimens, tissue and cell imaging, drug-effect studies, material science, and biotechnology research. Its imaging platform is suitable for live-cell imaging, cellular analysis, genetic engineering, molecular biology, and biopharmaceutical research workflows.

The microscope has been serviced and preventive maintenance has been completed. Optical quality is checked for field distortion and uniformity at the specimen plane, while fluorescence intensity is measured to verify light-source and filter performance. The listed configuration may be customized with additional objectives, filter cubes, fluorescence light sources, cameras, DIC or polarization components, and a motorized XY stage.