Sony LE-ID7000C Spectral Cell Analyzer, 5 Lasers, 147 Colors, 149 Detectors

The Sony LE-ID7000C Spectral Cell Analyzer is a high-parameter spectral flow cytometer configured with five excitation lasers, 147 fluorescence channels, and up to 149 detection parameters. Its spectral detector array supports high-dimensional multiparameter analysis for immunophenotyping, oncology research, immunology, stem cell analysis, vaccine studies, and translational biotechnology applications.

Optical and performance specifications

• Excitation lasers: 355 nm, 405 nm, 488 nm, 561 nm, and 637 nm
• Fluorescence channels: 147, using 32-channel photomultiplier tubes, single-channel photomultiplier tubes, and single-channel infrared-sensitive photomultiplier tubes
• Additional detection: Forward scatter (FSC) and side scatter (SSC)
• Fluorescence sensitivity: FITC ≤ 58 MESF, PE ≤ 4 MESF, APC ≤ 7 MESF
• Signal resolution: 20-bit height and 32-bit area
• Sampling rate: 60 MHz
• Event rate: Up to 40,000 events/second
• Particle detection: Approximately 160 nm
• Fluorescence resolution: CV < 3%

Fluidics and sample handling

• Sample flow rate of approximately 33–200 µL/min
• Prime, wash, purge, and decontamination cleaning modes
• Sheath tank and waste tank fluidics
• Automated sampler compatible with 96-well plates, 384-well plates, and 24 × 5 mL tubes
• Low carryover of <0.1%
• Automated sample mixing modes
• Minimum sample volume of approximately 10–100 µL, depending on format
• Ambient-to-4°C temperature control with a Peltier sample cooling module

Included equipment and accessories

• Sony LE-ID7000C Spectral Cell Analyzer
• Sony LE-ID7000 Fluidics Cart
• Sony 24-Tube Sample Rack for 12 × 75 mm (5 mL) tubes
• Sheath supply container with level sensor and tubing
• Waste container with level sensor and tubing
• Desktop PC running ID7000 software
• PC monitor, keyboard, and mouse
• Power and communication cables

The pre-owned instrument was made in 2023 and had five lasers with fewer than 100 hours of use at the time of testing. Testing included startup verification, ID-7000 software connection, fluidics cleaning and inspection, flow-rate calibration with Sony AlignCheck beads, sample-loader verification, agitation and cooling checks, pneumatic control testing, and performance validation with 8 Peak Beads.

A complimentary one-year subscription to the De Novo FCS Express 7 Research Use Only analysis software is included. The software supports flow cytometry data import, gating, statistical analysis, kinetic analysis, customizable visualization, heat maps, automated reporting, and compatibility with FCS, LMD, CSV, BD FACSDiva, Beckman Coulter, Accuri C6, SPADE, Cytobank, and TXT files.

This Sony spectral flow cytometer is suitable for high-parameter immune profiling, rare cell detection, multiplex biomarker discovery, stem cell and differentiation research, molecular diagnostics development, bioprocessing research, and other biotechnology workflows requiring high-dimensional single-cell analysis. Installation and setup by a Sony service team are required.