NanoCellect Biomedical Wolf G2 Benchtop Microfluidic Cell Sorter with CS1 Chiller-Stirrer

The NanoCellect Biomedical Wolf G2 Cell Sorter is an advanced benchtop microfluidic cell sorting system designed for gentle, low-pressure sorting of sensitive and primary cells. Featuring dual laser excitation at 488 nm (blue) and 561 nm (yellow), it enables precise fluorescence discrimination across a wide spectral range with multiple emission detectors for multiparametric flow cytometry. Its droplet-free, sterile cartridge-based sorting workflow preserves high cell viability and reduces contamination risks, making it ideal for single-cell workflows, molecular diagnostics, and biotechnology research.

Key technical specifications include:

• Optics:
• 488 nm laser with detectors at 525/50, 580/25, 620/50, 706/95, 760LP nm
• 561 nm laser with detectors at 580/25, 620/50, 706/95, 760LP nm
• Beam size: 20 x 90 µm
• Forward scatter (FSC) at 0° ±15°, back scatter (BSC) at 180° ±15°
• Fluidics:
• Sample tubes: 1.5, 2.0, and 5.0 mL; sheath fluid in 50 mL conical tubes
• Sheath fluid use: 9.6 mL/hour (PBS or buffer of choice)
• Flow rates: 24 µL/min sample, 160 µL/min sheath
• Flow cell size: 200 x 70 µm; channel diameter down to 70 µm
• Sample pressure < 2 psi to ensure gentle handling
• Performance:
• Scattering sensitivity < 1.5 µm resolving lymphocytes, monocytes, granulocytes
• Fluorescence sensitivity < 200 MESF FITC and < 250 MESF PE
• Analysis speed: 2,000 events/second; sorting speed: 200 events/second (1-way)
• CS1 Chiller-Stirrer accesssory:
• Stirring speed adjustable 50–300 rpm
• Temperature control 4–24 °C for sample stability

This cell sorter is widely adopted in biotechnology, molecular biology, synthetic biology, and clinical diagnostics labs for enrichment of fluorescently labeled cell subsets, isolation of rare cell populations, and high-viability sorting critical in biopharmaceutical production pipelines and bioprocessing applications. The compact, benchtop design supports sterile operation in biosafety cabinets and reduces contamination risk in biomanufacturing workflows.

The included chiller-stirrer accessory maintains uniform sample suspension and temperature, essential for highly sensitive cell populations used in gene editing and single-cell analytics. The system's cartridge-based, droplet-free sorting eliminates aerosol generation, making it suitable for sensitive live-cell imaging and fluorescence analysis preceding downstream molecular assays.

Please note, the instrument is sold as-is without software license activation; a manufacturer visit is required for software configuration. Power controller for the CS1 accessory is not included.

Primary keywords: microfluidic cell sorter, benchtop cell sorter, dual-laser cell sorter, sterile cartridge-based sorting Long-tail keywords: gentle cell sorting for sensitive cell types, droplet-free sorting for high cell viability, widely used in molecular diagnostics and synthetic biology research, essential for single-cell workflows and bioprocessing applications.