Millipore Cytek Guava EasyCyte 12HT Flow Cytometer with 3-Lasers and 13-Channel Detection

The Millipore Cytek Guava EasyCyte 12HT Flow Cytometer is a precision benchtop instrument designed for high-throughput multiparametric cell analysis, widely used in molecular diagnostics, immunophenotyping, and bioprocessing workflows. Featuring 3 solid-state lasers (405 nm violet, 488 nm blue, 642 nm red) and 13 fluorescence detection channels, it provides comprehensive multicolor fluorescence analysis with a dynamic range of 5 decades and precise cell counting capabilities without the need for reference beads.

Key technical specifications and capabilities include:

• Optics & Detection:
• 3 lasers: 405 nm, 488 nm, 642 nm
• 13 fluorescence channels distributed as Violet (5), Blue (4), Red (2) plus forward and side scatter for granularity and sizing
• Filters optimized for Blue-V (448/45 nm), Green-V (525/30 nm), Yellow-V (583/26 nm), Red-V (695/50 nm), NIR-V (785/70 nm), etc.
• Absolute particle sizing range: 0.2 – 60 µm
• Fluorescence precision: <4% CV for standardized beads

• Sample Handling & Fluidics:
• Automated sampling from 96-well plates, 0.5 mL and 1.5 mL tubes with sample mixing
• Fluidics driven by positive displacement pump, enabling stable flow rate between 7–70 µL/min
• Minimal sample volume requirements: 100–900 µL depending on format
• No sheath fluid required, reducing waste (~50 mL per 8 hours continuous use)

• Performance & Software Integration:
• Acquisition speed: 10–1000 events/second suitable for rapid multiparametric datasets
• Includes GuavaSoft software for streamlined instrument control and data acquisition
• Comes with a 1-year subscription to De Novo FCS Express 7 Analysis software (Research Use Only version), providing advanced data visualization, customizable reporting, and compatibility with various flow cytometry file formats (FCS, BD FACSDiva, Accuri, SPADE, Cytobank)
• Automated quality control demonstrated through easyCheck bead assays ensures reproducible CVs and instrument calibration before shipment

• Applications:
• Immunophenotyping of hematopoietic and immune cells
• Cell cycle and apoptosis analysis
• Viability assays and intracellular cytokine staining
• Drug screening and biomarker discovery in biotechnology and clinical research
• Essential for molecular diagnostics, gene editing assays, and synthetic biology workflows requiring multiplex fluorescence cytometry

• Physical Dimensions:
• Compact footprint: 25 cm × 51 cm × 61 cm (W × D × H)
• Weight: 31.8 kg (70 lbs), suitable for benchtop installation

The instrument undergoes comprehensive preventive maintenance and servicing including fluidics decontamination, laser output verification, detector calibration, and functional testing to ensure optimal performance and reliability. It is offered with technical support, remote installation, and training services, making it a robust choice for research institutions, biotechnology labs, and biopharmaceutical production pipelines requiring precise and efficient flow cytometry analysis.

By integrating this flow cytometer, laboratories benefit from rapid sample processing and multiparameter fluorescence detection critical for advanced cell biology, biomedical engineering, and molecular cloning research initiatives. Its compact design combined with automated sampling capabilities makes it a popular solution in high-throughput bioprocessing and translational medicine environments.