Luminex xMAP INTELLIFLEX Multiplex Flow Cytometry System for Biomarker Analysis

The Luminex xMAP INTELLIFLEX System is an advanced multiplex flow cytometry platform designed for simultaneous detection of up to 500 analytes in a single sample, making it essential for molecular diagnostics, biomarker quantification, and immunoassay multiplexing in biotechnology and clinical research.

• Fluidics: Features a 200 µm square cuvette flow channel, 2 µL/second sample injection rate, and adjustable sample uptake volumes between 10 µL and 200 µL. The sheath flow operates at 7.9 ± 0.9 mL/min with temperature viscosity compensation, and sheath pressure ranges from 8 to 13 psi during normal operation (15 psi max), ensuring stable and reproducible fluidics ideal for bioprocessing workflows.
• Optics: Equipped with a 637 nm diode CW laser (30-50 mW) for bead classification and a 532 nm diode-pumped solid-state laser (up to 50 mW) for reporter excitation. Detection employs avalanche photodiodes with temperature compensation and photomultiplier tubes with 16-bit A/D resolution, providing a dynamic detection range of ≥ 5.5 decades for fluorescence signals critical in synthetic biology and gene expression analysis.
• Microsphere Multiplexing: Enables classification accuracy ≥ 80% with overall microsphere misclassification ≤ 2% and well-to-well carryover below 4%. The system reliably detects fluorochromes such as Phycoerythrin (PE), facilitating high-fidelity multiplex immunoassays essential in biopharmaceutical production pipelines.
• Throughput and Compatibility: Reads 96-well plates in under 20 minutes and 384-well plates in less than 75 minutes. Integrated temperature control maintains 35–60°C via a heater block, supporting stable assay conditions for vaccine research and live-cell biomarker profiling. A barcode reader streamlines sample tracking and ensures accuracy in molecular cloning workflows.
• Automation & Support: Incorporates automated fluidics control and sheath pressurization for consistent operation, suitable for clinical diagnostics and neuroscience applications. The system includes remote installation support, training sessions, and warranty coverage backed by tested and serviced quality assurance.

This multiplex immunoassay analyzer is widely adopted in biotechnology, molecular biology, clinical research, and immunology studies, offering high sensitivity, reproducibility, and throughput for complex assay panels. It is critical in biomanufacturing, molecular diagnostics, and systems biology research requiring robust fluorescence-based detection and multiplex protein quantification.