Luminex xMAP INTELLIFLEX Multiplex Immunoassay Flow Cytometry System Unit 2

The Luminex xMAP INTELLIFLEX System Unit 2 is a sophisticated multiplex immunoassay platform combining flow cytometry, fluorescent microspheres, and advanced optics designed for sensitive and high-throughput biomarker, cytokine, and protein quantification.

• Multiplexing Capability: Detect up to 500 analytes per sample using bead-based multiplex assays, essential for molecular diagnostics and bioprocessing workflows.
• Optics and Lasers: Features a 637 nm diode laser for classification and a 532 nm diode-pumped solid-state laser (DPSS) for reporter detection, ensuring high-sensitivity fluorescence analysis critical in biotechnology research and synthetic biology applications.
• Fluidics System: Automated fluidics with stable sheath flow rate (7.9 ± 0.9 mL/minute) and sample injection rate of 2 μL/second for consistent and reproducible sample handling, supporting live-cell assays and protein quantification studies.
• Detection and Readout: 16-bit A/D resolution with photomultiplier detection over a broad dynamic range (≥ 5.5 decades), enabling precise measurement of immune and gene expression markers with minimal carryover (<4%).
• Temperature Control: Integrated heater block compatible with 96- and 384-well plates, maintaining operational temperatures between 35–60 °C, optimizing assay reproducibility in clinical and vaccine research contexts.
• Barcode Integration: Built-in barcode reader streamlines sample tracking and workflow efficiency, important for clinical diagnostics and large-scale biomanufacturing pipelines.

The system has undergone rigorous service and validation, including fluidics calibration, flow path cleaning, and functional verification of XYZ stage and barcode reader modules, ensuring reliability for complex multiplex immunoassay protocols widely adopted in molecular biology and biopharmaceutical research.

This unit supports key application areas such as clinical biomarker discovery, vaccine development, and gene expression profiling, making it critical in biotechnology research sectors including molecular cloning workflows, synthetic biology research, and biopharmaceutical production pipelines.

Keywords: multiplex immunoassay system, flow cytometry multiplexing, bead-based assay analyzer, fluorescence detection system, automated fluidics, biomarker quantification, cytokine analysis, clinical research instrument, biotechnology multiplex platform, molecular diagnostics equipment.