Luminex FlexMAP 3D Multiplexing System - High-Throughput Flow-Based Bead Reader for Multiplex Assays

The Luminex FlexMAP 3D Multiplexing System is a highly specialized flow-based bead reader designed to perform multiplex assays for simultaneous detection and quantification of up to 500 analytes per well. Utilizing advanced xMAP bead-based technology, this system supports high-throughput analysis in 96- and 384-well microtiter plates, maximizing sample throughput for molecular diagnostics, biomarker validation, and immunoassay development workflows critical in biotechnology and clinical research.

Key technical specifications include:

• Laser Configuration: Reporter laser at 532 nm paired with a classification laser at 638 nm for precise bead discrimination and signal detection.
• Detection System: Incorporates a photomultiplier tube with a 565–585 nm bandwidth and avalanche photodiodes with temperature compensation, ensuring sensitive and reproducible data acquisition.
• Microsphere Capability: Distinguishes between 1 to 500 unique xMAP microspheres within a single sample, facilitating complex multiplex panels essential in gene expression and cytokine profiling assays.
• Dynamic Range: Offers a broad 4.5 logarithmic unit dynamic range supporting quantitative accuracy across diverse analyte concentrations.
• Fluidics and Sample Handling: Equipped with two syringes and a sample injection rate of 2 μL/second, this system handles sample volumes from 10 to 200 μL efficiently with validated fluidic pathways and sheath pressure control to maintain operational consistency.
• Read Times: Approximately 20 minutes per 96-well plate and 75 minutes per 384-well plate, optimized for biopharmaceutical production pipelines and large-scale molecular cloning workflows.
• Dimensions and Weight: Compact footprint measuring 58.4 x 65.3 x 45.7 cm and weighing 91 kg, integrating optics, fluidics, and control electronics in an ergonomic design suitable for live-cell imaging laboratories and biotechnology environments.

Included with the system is a computer running the Xponent version 4.3 control software, facilitating user-defined routine creation, data export, and automated calibration and validation protocols. The instrument underwent comprehensive in-house servicing including cleaning, laser alignment verification, and performance calibration to ensure reliable operation.

The FlexMAP 3D is extensively used in:

• Cytokine profiling
• Biomarker validation
• Immunoassay development
• Gene expression analysis
• Vaccine response studies

These applications make it an indispensable tool for researchers in molecular diagnostics, synthetic biology, and bioprocessing, widely adopted for high-throughput multiplex immunoassay testing and critical in advanced biopharmaceutical production pipelines.

This pre-owned unit comes fully serviced, with warranty and after-sales technical support, making it a cost-effective solution for laboratories seeking a robust bead-based multiplexing platform compatible with demanding biotechnology and clinical research applications.