Sage Science PippinHT High-Throughput DNA Size Selection System for Next-Generation Sequencing

The Sage Science PippinHT is an automated high-throughput DNA size selection system designed for next-generation sequencing (NGS) library preparation. It processes up to 24 samples per run, supporting consistent and precise selection of DNA fragments for demanding molecular biology and genomics workflows.

Size selection is performed using pre-filled agarose gel cassettes, with a selectable fragment range of 100 bp to 1.5 kb, depending on the cassette type. Automated operation helps improve NGS library consistency while reducing manual handling and operator-related variability.

Applications include:

• Whole-genome sequencing
• RNA sequencing (RNA-Seq)
• Chromatin immunoprecipitation sequencing (ChIP-Seq)
• microRNA analysis
• DNA library preparation for genomics and biotechnology workflows

Specifications:

• Sample capacity: Up to 24 samples per run
• Size selection range: 100 bp–1.5 kb, cassette-dependent
• Cassette types: 0.75%, 1.5%, 2%, and 3% agarose
• Maximum DNA quantity: 1.5 µg per sample
• Minimum DNA quantity: 15 ng per sample
• Run time: Approximately 30 minutes per run
• Electrophoresis: 75 V, constant or pulsed
• Optical detection: 470 nm excitation, 525 nm emission
• Operating mode: Suitable for continuous operation
• Dimensions (H × W × D): Approximately 460 × 330 × 640 mm
• Weight: Approximately 9 kg
• Power supply: 100–240 V, 50/60 Hz

Included equipment:

• 2 rinse cassettes
• Calibration fixture
• USB stick
• 19-inch HD LED monitor
• Keyboard
• Mouse with mouse pad

The unit is described as new and unused, with serial number HT00611. It is suitable for high-throughput NGS workflows, genomic library construction, and biotechnology research requiring automated DNA fragment size selection.