Cytek Aurora Spectral Flow Cytometer, 4-Laser, 48-Channel, 51-Detector with ASL Loader

The Cytek Aurora spectral flow cytometer is a pre-owned, fully functional benchtop flow cytometry system manufactured in 2022. It combines four spatially separated lasers, 48 fluorescence detection channels, and 51 total detectors for high-parameter cellular analysis and real-time spectral unmixing.

Optical Configuration

• Laser configuration: 4-laser V/B/YG/R
• Laser wavelengths: 405 nm, 488 nm, 561 nm, and 640 nm
• Fluorescence channels: 48
• Total detectors: 51, including 48 fluorescence channels and 3 scatter detectors
• Violet detector module: 16 channels, unevenly spaced bandwidth from 420–829 nm
• Blue detector module: 14 channels, unevenly spaced bandwidth from 498–829 nm
• Red detector module: 8 channels, unevenly spaced bandwidth from 652–829 nm
• Yellow-green detector module: 10 channels, unevenly spaced bandwidth from 567–829 nm
• Forward scatter: High-performance semiconductor detector with a 488 nm bandpass filter
• Side scatter: Two high-performance semiconductor detectors with 405 nm and 488 nm bandpass filters
• Detection technology: CWDM semiconductor detector arrays for full-spectrum light capture
• Laser beam profile: Flat-top laser beams for stable excitation and consistent measurement

Fluidics and Sample Handling

• Vacuum-driven fluidics system
• Fluidics modes include Long Clean, SIT Flush, Purge Filter, Clean Flow Cell, and Fluidics Shutdown
• Manual sample input for 12 × 75 mm polystyrene and polypropylene tubes
• ASL Automated Sample Loader included
• 96-well plate and 96-deep-well plate compatibility
• 40-tube rack for 12 × 75 mm tubes
• Tube-mode flow rates: 15 µL/min low, 30 µL/min medium, and 60 µL/min high
• Plate-mode flow rates: 15 µL/min low, 30 µL/min medium, and 100 µL/min high
• Data acquisition rate up to 35,000 events/s

Included Equipment and Software

• Cytek Aurora Flow Cytometer
• ASL Automated Sample Loader
• 40-tube rack
• 96-well plate sampler
• Desktop PC running SpectroFlo software
• Two 4-liter fluid containers for sheath and waste solution
• Communication and power cables
• One-year subscription to De Novo FCS Express 7 Research Use Only (RUO) analysis software

The included analysis software supports FCS, LMD, CSV, BD FACSDiva, Beckman Coulter, Accuri C6, SPADE, Cytobank, and TXT file formats. FCS Express provides tools for gating, statistics, kinetic analysis, histograms, scatter plots, heat maps, data visualization, and report generation.

Applications

This high-parameter spectral flow cytometer is suitable for:

• Deep immunophenotyping of complex cell populations
• Rare immune subset detection in whole blood
• PBMC and tumor sample screening
• T-cell activation marker analysis
• Fluorescent protein expression profiling
• Cell biology, immunology, biotechnology, and molecular diagnostics research
• High-throughput cellular analysis in bioprocessing and biopharmaceutical research workflows

The four-laser architecture supports broad excitation coverage, autofluorescence extraction, and spectral unmixing for demanding multiparameter flow cytometry applications.

Service and Testing Information

The instrument was serviced and tested by an in-house technical team. Testing included startup verification, control software connection, fluidics cleaning and inspection, SIT calibration, flow cell cleaning cycles, sample loader operation, and quality control using SpectraFlo beads. Calibration bead results were reported within specification, laser power output was confirmed stable, and the system passed operational stress testing.