Agilent NovoCyte Penteon Flow Cytometer, 5 Lasers, 30 Colors, 32 Detectors with NovoSampler Q and Fluidics Station II

Agilent NovoCyte Penteon Flow Cytometer configured with five lasers, 30 fluorescence colors, and 32 detectors for high-sensitivity multiparameter cell analysis. The system includes an Agilent NovoSampler Q automated sampler and NovoCyte Fluidics Station II for tube and microplate workflows.

Configuration and optical performance

• Five solid-state lasers with onboard thermoelectric cooling:
• 349 nm UV laser, 20 mW
• 405 nm violet laser, 100 mW
• 488 nm blue laser, 100 mW
• 561 nm yellow laser, 100 mW
• 637 nm red laser, 100 mW
• Laser allocation: UV/7, violet/7, blue/6, yellow/6, and red/4
• 30 colors and 32 detectors
• Forward scatter and side scatter from the 488 nm blue laser
• Elliptical laser beam profile: 10 × 60 µm
• Silicon photomultiplier fluorescence detection with an individual photodetector for each channel
• FSC sensitivity: 0.4 µm
• SSC sensitivity: 0.1 µm
• Fluorescence threshold sensitivity:
• FITC ≤40 MESF
• PE ≤10 MESF
• APC ≤10 MESF
• Pacific Blue ≤30 MESF
• Fluorescence resolution: <3% CV for CEN

Fluidics and sample handling

• Sample acquisition rate: 100,000 events/second
• Positive-displacement syringe pump
• Volumetric absolute count precision: <5%
• Continuously adjustable sample flow rate: 5 to 120 µL/min
• Sheath flow rate: 6.5 mL/min
• Sample acquisition volume: 5 µL to 5 mL
• Manual sample formats: 12 × 75 mm tubes and 1.5 mL Eppendorf tubes
• NovoSampler Q compatibility:
• 40-tube rack for 12 × 75 mm tubes
• 24-well plates
• 48-well plates
• 96-well flat-, U-, and V-bottom microplates
• 384-well microplates
• Active fluid-level sensing with weight sensors and automated warnings
• Fluid container capacity:
• 3 L sheath
• 3 L waste
• 500 mL cleaning solution
• 500 mL rinse solution

Included equipment and software

• Agilent NovoCyte Penteon Flow Cytometer, identifier U7V7B6Y6R4
• Agilent NovoSampler Q
• Agilent NovoCyte Fluidics Station II
• Fluidics Station II bottles and caps, fluid harness, and cables
• Desktop computer with NovoExpress software trial version; license purchase required separately
• One-year subscription to De Novo FCS Express 7 Analysis Software, Research Use Only (RUO) version
• Communication and power cables
• NovoCyte Penteon Flow Cytometer manual

The included FCS Express software supports flow cytometry data import, gating, statistics, kinetic analysis, histograms, scatter plots, heat maps, automated reporting, and multi-format file handling. It supports formats including FCS, LMD, CSV, BD FACSDiva, Beckman Coulter, Accuri C6, SPADE, Cytobank, and TXT files.

Applications

• Immunophenotyping and immune cell population characterization
• Cell-surface and intracellular protein analysis
• Apoptosis, viability, and cell-health assessment
• Cell-cycle and proliferation analysis
• Cytokine production and functional immune-response studies
• Stem cell, cancer, and translational research
• Biomarker discovery and validation
• High-throughput clinical and research workflows
• Biotechnology, molecular biology, biopharmaceutical research, and cell-based assay development

This multiparameter flow cytometer is suitable for complex fluorescence panels, rare-cell detection, high-throughput sample processing, and quantitative cell analysis in biotechnology and biomedical research.

Condition and testing

• Made in 2021
• Fully functional pre-owned instrument
• Fluidics priming, startup, shutdown, cleaning, and decontamination sequences tested
• Autosampler calibration and operation verified
• Fluidics Station II sensors tested
• Baseline testing performed with multi-peak beads
• QC bead test passed
• Laser power stability confirmed
• Preventive maintenance performed before shipment