Molecular Devices CloneSelect Imager FL with DispenCell Single-Cell Dispenser and Lumencor Aura III

The Molecular Devices CloneSelect Imager FL with DispenCell Single-Cell Dispenser is an integrated laboratory system for single-cell imaging, precision dispensing, and monoclonality verification. The platform combines fluorescence and brightfield imaging with automated single-cell deposition for clonal cell-line development, biologics research, and gene-edited cell population screening.

CloneSelect Imager FL configuration

• High-resolution CMOS camera
• Imaging speed:
• White-light imaging of 96-well or 384-well microplates: less than 2 minutes
• Fluorescence imaging of 96-well or 384-well microplates: less than 8 minutes
• Resolution:
• High resolution: 1.8 microns
• Standard resolution: 3.6 microns
• Objective: 4x
• White-light transillumination source: 5 W xenon flash lamp
• Multichannel fluorescence source: Lumencor Aura III LED light engine
• Fluorescence channels: GFP/RFP
• Compatible source plates: 96-well and 384-well SBS microplates
• Source plate capacity: 1 plate

DispenCell Single-Cell Dispenser configuration

• Operating pressure: less than 0.1 psi
• Stage capacity: 2 plates, compatible with 96-well and 384-well formats
• Loading cart: 3 tube positions
• Plate compatibility:
• 96-well plates: 200 µL
• 384-well plates: 50 µL
• Minimum cell number: 100 cells
• Sample preparation time: 10 minutes
• 96-well plate dispensing time: 5 minutes
• Cell parameters: cell size and doublets
• Calibration: not required
• Cleaning: not required

The system supports automated clonal isolation workflows, including single-cell detection, tracking, selection, and deposition. It is suited to stable cell-line generation, monoclonality assessment, biologics development, cell viability studies, molecular biology research, and gene-editing workflows.

Included equipment comprises the CloneSelect Imager FL, DispenCell Single-Cell Dispenser, Lumencor Aura III, computer with CloneSelect software, monitor, keyboard, mouse, and power and communication cables. The pre-owned instrument was manufactured in 2022 and underwent functional testing, including software communication, objective and filter inspection, camera verification, multi-well image acquisition, Aura III operation, and full X- and Y-stage movement. The dispenser was powered on, initialized, connected to software, and subjected to a self-check; further dispensing tests require proprietary consumable tips.