Nexcelom Revvity Cellaca MX FL5 High-Throughput Fluorescence Cell Counter Cytometer

The Nexcelom Revvity Cellaca MX FL5 is a high-throughput fluorescence cell counter cytometer designed for rapid and quantitative cell analysis, essential in molecular diagnostics, bioprocessing, and biopharmaceutical production pipelines. This system integrates brightfield and 5-color fluorescence imaging for multiparametric cell assessments, widely adopted in synthetic biology and cellular research applications.

• Fluorescence Excitation Wavelengths: 365 nm, 470 nm, 527 nm, 620 nm
• Emission Filters: 452 nm, 534 nm, 605 nm, 655 nm, 692 nm
• Compatible with common viability and marker dyes including Trypan Blue, AO/PI, Hoechst, DAPI, PE, APC, FITC, CFDA, Calcein AM, and 7AAD
• High-throughput capacity: processes 24 samples in approximately 2.5 minutes, optimizing workflows for gene editing and cell line monitoring
• Minimal sample volume required: 25 µL per well (total well volume 50–200 µL)
• Detects cells ranging from 5 to 80 µm in diameter within a concentration range of 1x10^5 to 1x10^7 cells/mL
• Supports 96- and 384-well plate formats with onboard dilution and sample mixing functions
• Compact footprint: 33 x 33 x 41 cm; weight: 19 kg, ideal for laboratory benchtops
• Real-time viability and concentration metrics facilitate applications such as CAR-T cell therapy monitoring, PBMC analysis, and biomanufacturing quality control
• Utilizes a motorized stage and CMOS camera for automated plate scanning with brightfield and fluorescence imaging
• Engineered for precision with verified fluorescence excitation stability, emission filter clarity, and low electronic noise to ensure accurate and reproducible cell morphology and viability measurements

This instrument supports biotechnology research fields including molecular biology, cell therapy development, synthetic biology, and clinical diagnostics. It is critical in workflows requiring rapid and reliable cell counting, viability assessment, and multiparametric fluorescence analysis, making it a valuable tool for researchers and medical professionals engaged in complex cell-based assays.

The Cellaca MX FL5 is distributed with Matrix software for comprehensive data analysis and includes remote setup and training options. Its design enhances laboratory productivity while maintaining stringent quality controls required for bioprocessing and molecular cloning workflows.

Overall, this system is recognized for its high-throughput performance, versatility in fluorescence channels, and compatibility with diverse fluorescent dyes, making it indispensable in modern cell imaging and quantitative cell analysis applications.